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Using PCR degenerate primers, designed with reference to the sequences of the conserved amino acids of known expansins, to amplify cDNA fragments in litchi fruit by RT-PCR, two different cDNA fragments , named as Lc-Exp1 and Lc-Exp2 , were cloned. Lc-Exp1 and Lc-Exp2 was respectively composed of 531 bp encoding 177 amino acids and 537 bp encoding 179 amino acids. Eight cysteine residues and three tryp-tophan residues, which is supposed to be the characteristics of expansins, are conserved in both Lc-Exp1 and c-Exp2. In addition, the homology between the two expansins is 71. 6% at nucleotide acid sequences and 76.3% at amino acid sequences. The homology of Lc-Exp1 with Fa-Exp2 or Pp-Exp1 was 92.7% or 92.1%, but that of Lc-Exp2 with Fa-Exp2 or Pp-Exp1 was only 77. 4% or 76.3% at amino acid sequences.
Using PCR degenerate primers, designed with reference to the sequences of the conserved amino acids of known expansins, to amplify cDNA fragments in litchi fruit by RT-PCR, two different cDNA fragments, named as Lc-Exp1 and Lc-Exp2, were cloned. Lc-Exp1 and Lc-Exp2 were composed of 531 bp encoding 177 amino acids and 537 bp encoding 179 amino acids. Eight cysteine residues and three tryp-tophan residues, which are supposed to be the characteristics of expansins, are conserved in both Lc Expiration of Lc-Exp1 with Fa-Exp2 or Pp-Exp1 was 92.7% -Exp1 and c-Exp2 In addition, the homology between the two expansins was 71. 6% at nucleotide acid sequences and 76.3% at amino acid sequences. or 92.1%, but that of Lc-Exp2 with Fa-Exp2 or Pp-Exp1 was only 77.4% or 76.3% at amino acid sequences.