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目的探究复方蛹虫草(CCM)对高脂诱导的小肠炎症和H_2O_2与LPS诱导的IEC-6细胞凋亡的干预作用。方法C57BL/6J雄性小鼠随机分为正常对照组、模型组及CCM 90、180和540 mg·kg~(-1)·d~(-1)组。采用高脂饮食建立小鼠小肠炎症模型。造模同时ig给药,每天1次,连续8周,比色法测定TG、T-CHO、LDL-C、HDL-C;酶联免疫吸附测定法(ELISA)测定血清细胞炎性因子TNF-α、IL-1β、IL-6、LPS。HE染色法比较各组空肠、回肠组织病理改变,MTT方法检测H_2O_2与LPS诱导的IEC-6细胞存活率。结果与模型组相比,CCM各给药组T-CHO、LDL-C水平显著降低;小肠肠腺数目显著增加,黏膜层厚度接近正常组小肠组织,黏膜上皮细胞未见明显变性、坏死,炎性细胞消失;CCM给药组小鼠血清TNF-α、IL-1β、IL-6、LPS含量显著降低;CCM各组IEC-6细胞存活率显著增加且效果好于CME组和GBE组。结论 CCM可以改善高脂诱导的小鼠小肠炎症,其机理可能与其提高小肠上皮细胞的存活率有关。
Objective To investigate the effects of compound Cordyceps militaris (CCM) on the inflammation induced by high fat and the apoptosis of IEC-6 cells induced by H 2 O 2 and LPS. Methods C57BL / 6J male mice were randomly divided into normal control group, model group and CCM 90,180 and 540 mg · kg -1 · d -1 groups. The model of mouse intestinal inflammation was established by high fat diet. TG, T-CHO, LDL-C and HDL-C were determined by colorimetry. The serum levels of inflammatory cytokine TNF-α and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA) α, IL-1β, IL-6, LPS. HE staining was used to compare the pathological changes of jejunum and ileum in each group. The survival rate of IEC-6 cells induced by H 2 O 2 and LPS was detected by MTT assay. Results Compared with the model group, the levels of T-CHO and LDL-C in each group were significantly decreased; the number of intestinal small intestine was significantly increased; the thickness of mucosal layer was close to that of normal group; no obvious degeneration, necrosis and inflammation were found in mucosal epithelial cells The number of serum TNF-α, IL-1β, IL-6 and LPS in CCM-treated group was significantly decreased; the survival rate of IEC-6 cells in CCM groups was significantly increased and better than that of CME and GBE groups. Conclusion CCM can improve the inflammation induced by high fat in small intestine in mice and its mechanism may be related to its increasing the survival rate of intestinal epithelial cells.