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目的主要研究氟诱导成骨细胞MC3T3-E1发生自噬、凋亡及两者之间的关系。方法利用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)比色法检测氟对成骨细胞MC3T3-E1的增殖活性影响,探寻氟引起凋亡的浓度;流式细胞术检测细胞凋亡;western blot免疫印记技术检测凋亡相蛋白;利用western blot免疫印记技术检测Beclin 1蛋白及LC3蛋白表达水平及变化。结果氟能明显抑制成骨细胞MC3T3-E1增殖,诱发细胞凋亡,其抑制作用呈时间、剂量依赖性;氟在促进细胞凋亡的同时也促进自噬产生;3-甲基腺素(3-MA)抑制自噬后,氟诱导的凋亡进一步增多。结论氟明显抑制MC3T3-E1细胞增殖,同时诱发细胞凋亡和自噬;氟诱导的自噬部分拮抗其诱导的凋亡。
Objective To study the relationship between autophagy and apoptosis induced by fluoride-induced osteoblast MC3T3-E1 and their relationship. Methods The proliferative activity of osteoblasts MC3T3-E1 was examined by MTT colorimetric assay Flow cytometry was used to detect apoptosis. Western blot was used to detect the apoptotic protein. Western blot was used to detect the expression of Beclin 1 protein and LC3 protein. Results Fluorine could significantly inhibit the proliferation and induce the apoptosis of MC3T3-E1 cells in a time-and dose-dependent manner. Fluorine can promote the apoptosis of cells and promote the production of autophagy. 3-Methylglutamine (3 -MA) inhibition of autophagy, fluorine-induced apoptosis further increased. Conclusion Fluoride can significantly inhibit the proliferation of MC3T3-E1 cells and induce apoptosis and autophagy at the same time. Fluorine-induced autophagy partially antagonizes the induced apoptosis.