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目的在艾滋病病毒(HIV)感染早期,观察HIV-1特异性抗体依赖的细胞毒性作用(ADCC)与病程进展的关系。方法利用HIV-1特异性多肽刺激和多色流式技术,对前期采集的、感染时间在1年以内的男男性行为人群(MSM)标本进行ADCC检测。结果共采集HIV-1感染者标本58份,CD4+T淋病细胞计数与外周血病毒载量呈明显负相关(r=-0.281 6,P=0.032 3)。ADCC检测结果显示,病毒载量与HIV-1Pol特异性CD2+IFN-γ+细胞占CD3-细胞的比例呈显著的正相关(r=0.260 7,P=0.046 2);而病毒载量与HIV-1Gp120特异性CD2+CD107a+细胞占CD3-[A1]细胞的比例呈显著负相关(r=-0.342 9,P=0.009 0)。结论在HIV-1感染早期,感染者体内即可产生针对Pol和Env蛋白的ADCC反应,其中针对Gp120蛋白的ADCC反应具有控制病程进展的作用。
Objective To observe the relationship between HIV-1 specific antibody-dependent cytotoxicity (ADCC) and the course of disease in the early stage of HIV infection. Methods HIV-1-specific peptide stimulation and multi-color flow cytometry were used to detect ADCC in MSM specimens collected in the previous period and within 1 year. Results A total of 58 samples of HIV-1 infected patients were collected. The CD4 + T lymphocyte count was negatively correlated with the viral load of peripheral blood (r = -0.281 6, P = 0.032 3). The results of ADCC showed that there was a significant positive correlation between viral load and HIV-1Pol-specific CD2 + IFN-γ + cells in CD3- cells (r = 0.2607, P = 0.046 2) There was a significant negative correlation between -1Gp120-specific CD2 + CD107a + cells and CD3- [A1] cells (r = -0.342 9, P = 0.009 0). Conclusion In the early stage of HIV-1 infection, ADCC responses against Pol and Env proteins can be produced in infected individuals. The ADCC response to Gp120 protein has the effect of controlling the course of disease.