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贵州省卫生防疫站在探讨简易快速诊断丝虫病的方法中,经过反复实践,试制成功犬丝虫提纯抗原(FPT),并对319例丝虫病人进行了皮内试验,阳性率为91.2%。抗原的制备:自犬心取出犬恶丝虫成虫,用生理盐水洗净,将虫体置无菌滤纸上吸去水分,剪碎,用冷丙酮提取8次,每次15分钟,脱除脂肪,置干燥器内干燥后碾成粉末。每克干粉加25毫升0.1当量盐酸,在室温内搅拌提取48小时后取出,放入每分钟3000转速的离心器离心10分钟,沉淀物再用
In the method of quick and easy diagnosis of filariasis, Guizhou Provincial Health and Anti-Epidemic Station, after repeated practice, tried to produce FPT, and 319 cases of filariasis were tested by intradermal test, the positive rate was 91.2% . Antigen preparation: Dog heartworm removed from the dog heart adults, washed with saline, the parasitic sterile filter paper to absorb moisture, cut, extracted with cold acetone 8 times, each 15 minutes, remove fat , Set dryer dries into powder. Add 25 ml of 0.1N hydrochloric acid to each gram of dry powder, remove by stirring at room temperature for 48 hours, centrifuge at 3000 rpm for 10 minutes, recycle the pellet