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[目的]研究Hg2+对小麦种子萌发和幼苗生长的影响。[方法]以郑州9023为供试材料,采用室内水培方法,研究不同浓度(0.025、0.050、0.100、0.200、0.300、0.400、0.500 mmol/L)Hg2+溶液浸种处理后,对小麦发芽率及幼苗苗高、根长、苗鲜重及叶片、根和萌发种胚中过氧化物酶(POD)、淀粉酶活性等的影响。[结果]低浓度Hg2+(≤0.100 mmol/L)处理对小麦种子萌发、幼苗苗高、根长和苗鲜重的影响较小;在Hg2+浓度>0.100 mmol/L时,对小麦种子萌发和幼苗的正常生长有明显抑制作用。低浓度Hg2+(≥0.025 mmol/L)可诱导小麦幼苗叶片、根和萌发种胚中POD活性明显升高,而抑制淀粉酶活性使之明显下降,并且这种影响随着Hg2+浓度的增大而增强。[结论]Hg2+胁迫可以改变小麦幼苗叶片、根和萌发种胚中POD和淀粉酶活性,从而影响其膜脂氧化正常代谢,导致生长所需的能量和底物的供应不足,最终抑制了幼苗的生长。
[Objective] The research aimed to study the effect of Hg2 + on the seed germination and seedling growth of wheat. [Method] With Zhengzhou 9023 as test material, indoor hydroponic method was used to study the germination rate and seedling growth of wheat (Triticum aestivum L.) treated with Hg2 + solution of different concentrations (0.025,0.050,0.100,0.200,0.300,0.400,0.500 mmol / L) Seedling height, root length, seedling weight and peroxidase (POD), amylase activity in leaves, roots and germinating embryos. [Result] The effects of low concentration of Hg2 + (≤0.100 mmol / L) on the seed germination, seedling height, root length and seedling weight of wheat were little. When Hg2 + concentration was> 0.100 mmol / L, the seed germination and seedling The normal growth has a significant inhibitory effect. Low concentrations of Hg2 + (≥0.025 mmol / L) induced a significant increase in POD activity in leaves, roots and germinating embryos while significantly decreased the activity of amylase, and as the concentration of Hg2 + increased Enhanced. [Conclusion] Hg2 + stress could change the POD and amylase activities in leaves, roots and germinating embryos of wheat seedlings, thus affecting the normal metabolism of lipid peroxidation, resulting in the energy needed for growth and the supply of substrate, eventually inhibiting the seedlings Grow.