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目的比较不同工艺芪葵颗粒对糖尿病肾病模型大鼠的保护作用。方法选用健康SD鼠54只,雄性,8周龄。采用高糖高脂饲料+单侧肾脏结扎术+腹腔注射STZ(40 mg/kg)的方法建立糖尿病肾病模型,造模成功后将其分为五组:模型组、厄贝沙坦组、工艺1组(水煮浓缩方)、工艺2组(水煮醇提浓缩方)、工艺3组(水提醇提浓缩合并方),另设空白组。三种不同工艺芪葵颗粒以相同剂量18.67 g/kg分别于造模成功后连续给药5周,观察不同工艺芪葵颗粒对糖尿病肾病大鼠血糖、尿蛋白、肌酐、内生肌酐清除率的影响,并行病理组织学检查。结果与空白组比较,模型组大鼠体重显著降低(P<0.01),进食量、饮水量、尿量明显增加(P<0.05或P<0.01),血糖明显升高(P<0.01)。给药干预后,与模型组比较,各给药组体重不同程度增加,进食量、饮水量、尿量不同程度减少;工艺2组和工艺3组血糖明显降低(P<0.05);厄贝沙坦组和工艺3组血肌酐明显降低(P<0.05或P<0.01);各给药组内生肌酐清除率明显升高(P<0.05或P<0.01);厄贝沙坦组以及工艺2、3组尿蛋白排泄率明显降低(P<0.01或P<0.05)。病理组织学结果显示,模型组大鼠肾脏主要表现为肾小球肥大、肾小管扩张、间质及肾盂炎细胞灶性浸润;与模型组比较,各给药组肾脏病变范围和程度略有减轻,以厄贝沙坦组和工艺2组肾小球和肾小管病变减轻略为明显。结论不同工艺芪葵颗粒对糖尿病肾病的保护作用与降低血糖、肌酐和尿蛋白排泄和减轻肾脏病变有关。以工艺2组与工艺3组较为明显。
Objective To compare the protective effects of Qikui granule on diabetic nephropathy model rats. Methods 54 healthy SD rats were selected, male, 8 weeks old. Diabetic nephropathy model was established by high-fat and high-fat diet + unilateral renal ligation + intraperitoneal injection of STZ (40 mg / kg). The models were divided into five groups: model group, irbesartan group, 1 group (boiled concentrated side), process 2 (water boiled alcohol mention concentrated side), process 3 (water extract alcohol condensation and side), the other a blank group. Three different kinds of Qiguoi granules were administrated continuously at the same dose of 18.67 g / kg for 5 weeks after successful modeling. The effects of Qiguoi granules on blood glucose, urine protein, creatinine and creatinine clearance in diabetic nephropathy rats were observed Influence, parallel histopathological examination. Results Compared with the blank group, the body weight of model rats decreased significantly (P <0.01), the food intake, water intake and urine volume increased significantly (P <0.05 or P <0.01) and the blood glucose increased significantly (P <0.01). Compared with the model group, the body weight of each administration group increased to varying degrees, the amount of food intake, water intake and urine output decreased to varying degrees after treatment. The blood glucose of the second and third groups were significantly decreased (P <0.05) (P <0.05 or P <0.01). The clearance rate of endogenous creatinine in each group was significantly increased (P <0.05 or P <0.01). The irbesartan group and the treatment 2 , Urinary protein excretion rate was significantly lower in the three groups (P <0.01 or P <0.05). Histopathological results showed that the kidneys in model group mainly showed glomerulus hypertrophy, tubule dilatation and focal infiltration of glomerulonephritis cells. Compared with model group, the extent and extent of renal lesions in each group were slightly relieved , To irbesartan group and process group 2 glomerular and tubular lesion was slightly reduced. Conclusion The protective effect of different process Qiguoi granules on diabetic nephropathy is related to the reduction of blood glucose, creatinine and urinary protein excretion and the alleviation of renal lesions. The second group with the process group 3 more obvious.