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目的建立高仿真燃煤型大鼠氟斑牙动物模型。方法以高氟煤拌粘土为燃料复制疫区空气环境,病区煤烘玉米配制饲料,30只SD大鼠按雌雄各半体重均等随机分为5组,即高氟空气饲养室内给予食物氟40 mg/kg(H)、25 mg/kg(M)、10 mg/kg(L)、2.1 mg/kg(A)组,阴性对照(C)组,观测大鼠体重、氟斑牙发生情况、尿氟及血氟含量、下切牙牙冠长度及釉质表面的超微结构。结果生火期空气氟浓度为(28.5±5.3)μg/m3、闭火期为(4.1±1.2)μg/m3。高氟空气环境下各组雌、雄大鼠体重增长均较C组缓慢(P<0.05)。H、M组大鼠下切牙釉质出现白色条纹和白垩色斑等变化,尿氟、血氟含量较C组升高(P<0.05)。H、M组下切牙冠长度增长较C组慢(P<0.05)。釉质表面白垩色区域矿化不全、釉柱间隙增宽。结论高氟空气环境对大鼠的生长发育可能会产生一定影响,通过模拟高氟空气环境并饲予煤烘玉米成功复制了高仿真的燃煤型氟斑牙动物模型。
Objective To establish a rat model of high-fidelity coal-fired rat dental fluorosis. Methods The high-fluorine coal-blended clay was used as fuel to copy the air environment in the epidemic area. The wadding corn was used to prepare the feed. Thirty SD rats were randomly divided into five groups according to the weight of each male and female. The body weight and the incidence of dental fluorosis in rats were observed and the control group rats were divided into two groups: control group (mg / kg), 25 mg / kg (M), 10 mg / Urinary fluoride and blood fluoride content, incisor crown length and enamel surface ultrastructure. Results During the firing period, the fluorine concentration in the air was (28.5 ± 5.3) μg / m3 and that in the closed phase was (4.1 ± 1.2) μg / m3. The weight gain of female and male rats in each group was higher than that in group C under high fluoride atmosphere (P <0.05). There were white streaks and chalky spots on the incisors of enamel in H and M groups, and the content of urinary fluoride and blood fluoride in C group was higher than that in C group (P <0.05). H, M group lower incisors crown length growth slower than the C group (P <0.05). Enamel surface chalky mineralization incomplete, glaze column widening. Conclusion The high fluoride air environment may have an impact on the growth and development of rats. The high simulation animal model of coal-based dental fluorosis has been successfully replicated by simulating the high-fluoride air environment and feeding coal to bake corn.