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目的探讨促红细胞生成素( erythropoietin, EPO) 进行预处理对全脑缺血大鼠学习记忆能力的影响。方法采用 4- VO 法制作全脑缺血模型, 将 SD 大鼠分为假手术组、生理盐水组和 EPO 组。生理盐水组和EPO 组于术前 3 h, 分别脑室立体定向注射生理盐水和重组人促红细胞生成素( rHu- EPO) , 假手术组只进行假手术处理。观察缺血后 72 h 海马细胞凋亡和缺血后 4 周大鼠学习和记忆力的变化。结果缺血后 72 h, EPO组海马 CA1 区较生理盐水处理组有较少的凋亡细胞(P <0.01), 并且缺血 4 周后, EPO 组学习记忆能力明显高于生理盐水组(P <0.01)。结论 EPO 预处理可以抑制缺血海马神经元凋亡及减轻全脑缺血造成大鼠学习和记忆力的损害。
Objective To investigate the effects of erythropoietin (EPO) pretreatment on learning and memory abilities in rats with global cerebral ischemia. Methods The model of global cerebral ischemia was established by 4-VO method. SD rats were divided into sham operation group, saline group and EPO group. Normal saline and recombinant human erythropoietin (rHu-EPO) were injected stereotacticly into ventricles at 3 h before operation in sham and EPO groups, respectively. Sham-operated rats were sham-operated only. The changes of hippocampal apoptosis and learning and memory of rats at 72 h after ischemia were observed. Results At 72 h after ischemia, there were fewer apoptotic cells in hippocampal CA1 area of hippocampus in EPO group (P <0.01), and the learning and memory ability of EPO group was significantly higher than that of saline group (P <0.01). Conclusion EPO pretreatment can inhibit the apoptosis of ischemic hippocampal neurons and alleviate the learning and memory impairment of rats caused by global cerebral ischemia.