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目的:研究盐酸氮芥(HN2)对青春前期小鼠睾丸组织的氧化应激损伤及叶下珠植物(PU)提取物对损伤的保护作用。方法:将64只小鼠随机分为A组(生理盐水对照组)、B组(1.25mg/kgHN2)、C组(2.5mg/kgHN2)、D组(5mg/kgHN2)、E组(5mg/kgHN2+125mg/kgPU)、F组(5mg/kgHN2+250mg/kgPU)、G组(5mg/kgHN2+500mg/kgPU)、H组(500mg/kgPU),每组8只,按各组PU剂量灌胃,qd×5d,d5一次性腹腔给予相应的HN2。24h后制备各组小鼠睾丸组织匀浆,检测还原型谷胱甘肽(GSH)含量、谷胱甘肽S转移酶(GST)及谷胱甘肽还原酶(GR)酶活性。结果:HN2处理后,睾丸内GSH含量、GST及GR酶活性均随着HN2剂量增加而降低。5mg/kgHN2处理的同时,随着PU剂量增加,睾丸内GSH含量、GST及GR酶活性增加。与PU未干预的高剂量HN2组比较,不同剂量PU干预5mg/kgHN2后,睾丸内GST含量、GST及GR酶活性均显著增加(P<0.01)。与A组比较,F及G组的GSH含量、GST及GR酶活性无显著性差异(P>0.05),H组睾丸内GSH含量、GST及GR酶活性均无显著变化(P>0.05)。结论:HN2通过氧化应激损伤睾丸组织,PU可有效缓解该毒性作用。
OBJECTIVE: To study the protective effects of hydrogen mustard (HN2) on oxidative stress in testes of prepubertal mice and extracts of Phyllanthus urinaria (PU). METHODS: Sixty-four mice were randomly divided into group A (saline control group), group B (1.25 mg/kg HN2), group C (2.5 mg/kg HN2), group D (5 mg/kg HN2), and group E (5 mg/ kgHN2+125mg/kgPU), F group (5mg/kgHN2+250mg/kgPU), G group (5mg/kgHN2+500mg/kgPU), H group (500mg/kgPU), 8 in each group, according to the PU dose of each group Stomach, qd×5d, d5 were administered intraperitoneally to the corresponding HN2. After 24 hours, testicular tissue homogenates were prepared in each group to determine the content of reduced glutathione (GSH), glutathione S-transferase (GST) and Glutathione reductase (GR) enzyme activity. RESULTS: After HN2 treatment, the content of GSH, GST and GR enzyme activity in the testis decreased with increasing HN2 dose. Simultaneously with 5mg/kg HN2 treatment, GSH content, GST and GR enzyme activity in testis increased with increasing PU dose. Compared with the high-dose HN2 group without PU intervention, the GST content, GST and GR enzyme activity in testis were significantly increased after different doses of PU intervention with 5mg/kg HN2 (P<0.01). Compared with group A, there was no significant difference in GSH content, GST and GR enzyme activity between group F and group G (P>0.05). There was no significant change in GSH content, GST and GR enzyme activity in group H (P>0.05). Conclusion: HN2 damages testicular tissue through oxidative stress. PU can effectively relieve this toxic effect.