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目的探讨微卫星改变分析方法对中国人膀胱肿瘤诊断的可行性;期望能筛选出用于临床膀胱肿瘤患者诊断及术后监测的较好的微卫星位点组合。方法应用PCR-SSLP银染方法,先后选择6个、10个微卫星位点组织,分别对31、32例膀胱癌患者的肿瘤组织。结果31例膀胱癌中,28例微卫星改变阳性,其敏感性为90.3(28/31);32例膀胱癌中,30例微卫星改变阳性,其敏感性为93.75%(30/32),非膀胱移行细胞癌患者标本的微卫星改变为阴性,特异性为100%。微卫星改变与膀胱癌的分期、分级无相关性。结论微卫星改变分析方法对膀胱癌病人诊断及术后监测是一种灵敏有效的分子生物学方法。不同种族的微卫星位点组合诊断膀胱肿瘤的敏感性不同。新的6个微卫星位点组合可能在中国人膀胱肿瘤检测中是一种有效的检测方法。
Objective To investigate the feasibility of microsatellite alteration analysis for the diagnosis of bladder cancer in Chinese patients and to find a better combination of microsatellite loci for the diagnosis and postoperative monitoring of patients with clinical bladder cancer. Methods PCR - SSLP silver staining method was used to select 6 and 10 microsatellite loci, respectively, 31,32 cases of bladder cancer in patients with tumor tissue. Results Of the 31 cases of bladder cancer, 28 cases were microsatellite-positive and their sensitivity was 90.3 (28/31). Of the 32 cases of bladder cancer, 30 cases were microsatellite-positive and their sensitivity was 93.75% (30/32) Microsatellite DNA changes in non-bladder transitional cell carcinoma patients were negative with a specificity of 100%. Microsatellite changes and bladder cancer staging, grade no correlation. Conclusion The method of microsatellite alteration analysis is a sensitive and effective molecular biological method for the diagnosis and postoperative surveillance of bladder cancer patients. Different races of microsatellite loci in the diagnosis of bladder tumors with different sensitivities. The new combination of 6 microsatellite loci may be an effective detection method in Chinese bladder cancer detection.