论文部分内容阅读
目的了解丽水市遂昌县铜绿假单胞菌(PA)的耐药情况,分析其携带耐药基因的情况。方法选择2015年1至7月在遂昌人民医院不同来源的69株PA,:药敏试验采用纸片扩散(K-B)法,根据药敏统计结果,分析该地区的耐药情况,然后用PCR扩增法检测耐药铜绿假单胞菌(PA)携带耐药基因的情况。结果 69株PA中57菌株检出TEM基因,32株菌株检出有CARB基因,3株有GES基因;69株PA菌株对药物的耐药率情况为:亚胺培南13%,阿莫西林/克拉维酸10%,美罗培南17.4%,氨苄西林/舒巴坦97.1%,哌拉西林/他唑巴坦18.8%,替卡西林/克拉维酸46.4,头孢哌酮/舒巴坦95.7%,结论 69株PA菌株中,携带TEM和(或)CARB基因可能导致PA耐β内酰胺类抗菌药。
Objective To understand the drug resistance of Pseudomonas aeruginosa (PA) in Suichang County of Lishui City and analyze the situation of resistance genes carrying it. Methods Sixty-nine PA patients from different sources in Suichang People’s Hospital from January to July 2015 were selected. Drug-susceptibility test was performed by disk diffusion (KB) method. According to drug susceptibility statistics, the drug resistance in this area was analyzed. Detection of resistance to drug-resistant Pseudomonas aeruginosa (PA) by amplification assay. Results The TEM gene was detected in 57 isolates of 69 PA strains, CARB gene was found in 32 isolates and GES gene in 3 isolates. The drug resistance rate of 69 isolates to PA strains was: imipenem 13%, amoxicillin / Clavulanic acid 17.4%, ampicillin / sulbactam 97.1%, piperacillin / tazobactam 18.8%, ticarcillin / clavulanic acid 46.4, cefoperazone / sulbactam 95.7% CONCLUSION: Carrying TEM and / or CARB gene in 69 strains of PA may lead to PA beta-lactam antibiotics.