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为探明高温胁迫对不同产地茅苍术叶绿素荧光动力学参数的影响,采用土培盆栽法种植采集于英山、罗田、信阳和句容的茅苍术,于第一茬花开前进行高温胁迫处理,对其叶绿素荧光特性的变化进行观测和分析。结果表明,英山、罗田、信阳和句容组茅苍术开花前的叶绿素含量均随胁迫时间的延长而不断下降,7 d时,分别下降为对照的49.57%、52.21%、34.72%和37.35%,总体表现为:罗田>英山>句容>信阳。随着高温胁迫时间的延长,不同产地茅苍术的最大光化学量子产量(Fv/Fm)、PSII潜在光化学活性(Fv/Fo)、有效光化学量子产量(Fv’/Fm’)、光化学猝灭系数(q P)和表观光合电子传递速率(ETR)均呈下降趋势,且各指标均与胁迫时间呈负相关,英山组各参数下降幅度最大,而罗田组各参数总体水平较高;高温胁迫对茅苍术叶片Fv/Fo的影响比Fv/Fm大。非光化学猝灭系数(NPQ)呈先上升后下降趋势,信阳、英山组于1 d达最大值,罗田、句容组于3 d达最大值。研究表明,高温(40℃)对茅苍术叶绿素荧光特性有较大的影响,罗田组在胁迫过程中各参数均处于较高水平,抗高温能力表现较佳。
To investigate the effect of high temperature stress on the chlorophyll fluorescence parameters of different plants, we cultivated the plants of Atractylodes macrocephala which were collected from Yingshan, Luotian, Xinyang and Jurong by soil culture and pot culture, The changes of chlorophyll fluorescence characteristics were observed and analyzed. The results showed that the chlorophyll contents of Atractylodes, Luotian, Xinyang and Jurong groups before flowering decreased continuously with the prolongation of stress time, and decreased to 49.57%, 52.21%, 34.72% and 37.35 %, The overall performance is: Luotian> Yingshan> Jurong> Xinyang. With the prolonging of high temperature stress, the maximum photochemical quantum yield (Fv / Fm), the potential photochemical activity of PSII (Fv / Fo), the effective photochemical quantum yield (Fv ’/ Fm’) and the photochemical quenching coefficient q P) and the photosynthetic electron transfer rate (ETR) showed a downward trend, and each index was negatively correlated with the stress time, the largest decline in the parameters of the British Mountain Group, and the overall level of the parameters of the Luotian group higher; The effect of stress on the Fv / Fo of Atractylodes macrocephala was larger than that of Fv / Fm. The non-photochemical quenching coefficient (NPQ) firstly increased and then decreased, while that of Xinyang and Yingshan groups reached the maximum at 1 d, and reached the maximum at 3 days in Luotian and Jurong groups. The results showed that high temperature (40 ℃) had a significant effect on the chlorophyll fluorescence characteristics of Atractylodes lancea. The parameters of Luotian were all at a high level during stress and the performance of high temperature resistance was better.