,miR-129-5p suppresses proliferation, migration, and induces apoptosis in pancreatic cancer cells by

• 作 者: Zhisheng Qiu Xiaochun Wang Yuping Shi Mingxu Da
• 机 构: Department of Surgical Oncology, Gansu Provincial Hospital, Lanzhou 730000, China“,”Department of Ga
• 出 处: 生物化学与生物物理学报（英文版）
• 发表日期: 2019年10期
• 关键词: pancreatic cancer miR-129-5p PBX3 proliferation migration

Pancreatic cancer (PC) is the seventh most frequent cause of cancer-related deaths worldwide with a high mortality.MicroRNAs (miRNAs) act as important regulators for the development of PC and participate in the progression of PC.miR-129o5p was reported to regulate the progression of tumors,such as thyroid cancer and gastric cancer.However,the function of miR-129-5p in PC is still unclear.In this study,the down-regulation of miR-129-5p was detected in PC tissues and PC cells.miR-129-5p was overexpressed or knocked down in AsPC-1 and BxPC-3 cells.The results showed that miR-129-5p overexpression suppressed proliferation,migration and invasion,and induced apoptosis of PC cells,whereas miR-129-5p knockdown showed opposite effects.In addition,we found that pre-B-cell leukemia homeobox 3 (PBX3) overexpression promoted proliferation,migration and invasion,but reduced apoptosis of PC cells.PBX3 was identified as a target of miR-129-5p by informatics analysis and dual luciferase reporter assay.Finally,our results indicated that miR-129-5p suppressed cell proliferation and migration by targeting PBX3.This study demonstrated that miR-129-5p could function as a tumor suppressor in the progression and development of PC by targeting PBX3,providing a reliable prognostic factor and a new therapeutic strategy for PC.