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目的建立并评价PCR－SSCP检测结核分支杆菌耐药性基因突变的方法。方法根据katG基因易变区设计一对引物，PCR扩增，产物经沸点断裂成单键，经SDS－PAGE，比较电泳的位置。结果PCR检测结核菌DNA的灵敏度达到100个细菌／ml，与其它细菌和其他分支杆菌无交叉反应。30株敏感株和H37Rv的PCR产物经SSCP检测正常，20株耐异烟肼结核菌中，19株的PCR产物SSCP检测游异常电泳带，敏感性达95％。15株经DNA测序有点突变的菌株，全部出现异常电泳带，表明测序的结果与SSCP检测的结果有很好的一致性。结论SSCP技术是检测结核分支杆菌katG基因突变的较好方法。
Objective To establish and evaluate PCR-SSCP method for the detection of Mycobacterium tuberculosis resistance gene mutations. Methods A pair of primers was designed according to the variable region of katG gene. The product was amplified by PCR and broken into single bond by boiling point. SDS-PAGE was used to compare the electrophoresis positions. Results The sensitivity of PCR for detection of M. tuberculosis DNA reached 100 bacteria / ml, no cross-reaction with other bacteria and other mycobacteria. PCR products of 30 sensitive strains and H37Rv were detected by SSCP. Among the 20 strains of isoniazid-resistant Mycobacterium tuberculosis, 19 strains of PCR products were detected by SSCP. The sensitivity was 95%. Fifteen strains mutated slightly by DNA sequencing all showed abnormal electrophoresis bands, indicating that the sequencing results were in good agreement with the results of SSCP. Conclusion SSCP technique is a good method to detect the mutation of katG gene of Mycobacterium tuberculosis.