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本文证明在希尔反应速度饱和的强闪光下,消除膜两侧质子浓度差的解联剂也影响毫秒级延迟发光的快相。在对磷酸化解联程度相仿时,尼日利亚菌素对快相的消除作用大于氯化铵。光系统Ⅱ至质醌间的电子传递抑制剂同时降低快相及慢相延迟发光,抑制还原的质醌氧化的二溴百里香醌或不加电子受体使光合电子传递末端受阻则只降低慢相,而对快相影响很少。这表明影响快相的质子浓度差与膜联系紧密,且主要与光系统Ⅱ水氧化时的质子释放有关。
This paper shows that the solvating agent that eliminates the difference in proton concentration on both sides of the membrane also affects the fast phase of the delayed microsecond delay at a strong flash saturated with a Hill reaction. When the degree of dissociation of phosphorylation is similar, the elimination effect of Nigermycin on the fast phase is greater than that of ammonium chloride. Photo-system II to p-quinone electron transport inhibitors reduce both the fast phase and the slow-phase delayed luminescence, whereas the reduced quinone-oxidized dibromothymol or the blocked electron donor block the photosynthetic electron-transport end only to slow the slow phase , While little impact on the fast phase. This indicates that the difference of proton concentration affecting the fast phase is closely related to the membrane and mainly related to the proton release when the photosystem II is oxidized.