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根据已报道的马铃薯卷叶病毒基因组序列，设计合成一对特异性引物。以马铃薯卷叶病毒中国分离株（ＰＬＲＶ－Ｃｈ）的ＲＮＡ为模板，反转录合成了ｃＤＮＡ第一条链，经ＰＣＲ扩增后克隆于ｐＵＣ１９质粒中。进一步用ＰＣＲ鉴定，限制酶切分析和序列分析。结果表明，ＰＬＲＶ－Ｃｈ５６ｋＤ蛋白基因由１５２７个核苷酸组成，编码５０８个氨基酸，３＇端非编码区由１４１个核苷酸组成，与国外报道的苏格兰ＰＬＲＶ－Ｓ、荷兰ＰＬＲＶ－Ｎ、加拿大ＰＬＲＶ－Ｃ、澳大利亚ＰＬＲＶ－Ａ各株系核苷酸序列和氨基酸序列有很高的同源性。５６ｋＤ蛋白基因核苷酸同源率分别为９７．１％、９７．８％、９７．１％和９３．９％，推测的氨基酸同源率分别为９６．２％、９６．４％、９５．８％和９４．６％，非编码区核苷酸同源率分别为９７．９％、９７．２％、９８．６％和９８．６％。
According to the published sequence of potato leafroll virus genome, a pair of specific primers were designed and synthesized. The first strand of cDNA was reverse transcribed using the RNA of Potato Leafroll Virus Chinese isolate (PLRV-Ch) as a template and cloned into pUC19 plasmid by PCR amplification. Further identification by PCR, restriction analysis and sequence analysis. The results showed that the PLRV-Ch56kD protein gene consisted of 1527 nucleotides and encoded 508 amino acids, while the 3’-terminal non-coding region consisted of 141 nucleotides. Compared with the reported PLRV-S in Scotland, PLRV-N in the Netherlands, PLRV-C, Australia PLRV-A strains of nucleotide and amino acid sequences have very high homology. The nucleotide homology of 56kD protein gene was 97.1%, 97.8%, 97.1% and 93.9%, respectively. The deduced amino acid homologies were 96.2%, 96.4%, 95% .8% and 94.6% respectively. The nucleotide homology of non-coding region was 97.9%, 97.2%, 98.6% and 98.6% respectively.