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以DGD(diethylene glycol distearate)包埋、去包埋剂制片法,结合常规电镜制备术,观察研究了小麦珠被组织中胞间连丝的结构与分布特征及其与微丝的关系。试验结果表明,同层珠被细胞间壁上胞间连丝的分布密度比上下层珠被间的要显著地高得多;珠被中胞间连丝保持固有的正常结构,孔径30—40 nm,变动幅度不大,观察不到如作者以前在小麦珠心和胚乳中所见的那种胞间连丝经结构修饰而扩大、转变为开放态的景象。对去包埋剂制片的观察进一步揭示,从胞质骨架网络表层有纤细微丝以分散状向外伸展突入分界壁,时而可见各个微丝横贯分界壁而连接相邻骨架网络的图象。联系珠被中正常胞间连丝结构的稳定性与各个微丝以分散状跨越分界壁,讨论了微丝参与胞间连丝结构的可能性与可能模式。
The structure and distribution characteristics of intercellular filaments and their relationship with microfilaments were observed and studied with DGD (diethylene glycol distearate) embedding, embedding agent preparation and conventional electron microscopy. The results showed that the density of interplasia on the same layer of intercellular cells was significantly higher than that of the upper and lower layers of intergranular beads; , The change range is not big, can not observe as the author in the wheat nibs and endosperm seen in the kind of cell-like filaments by the structural modification and expansion, into the open state of the scene. The observation of embedding agent preparation further revealed that the fine microfilaments from the surface of cytoplasmic scaffold network spread outwards into the demarcation wall in a dispersed state, and it can be seen that each microfilament traverses the demarcation wall to connect with the image of the adjacent scaffold network. The relationship between the stability of normal interplanar filaments in the beads and the individual microfilaments scattered across the boundary wall was discussed. The possibility and possible mode of microform involvement in the intercellular silk structure were also discussed.