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目的肾髓质渗透梯度的形成与髓襻走行及转运功能关系密切,本研究拟建立长髓袢肾单位在髓质的走行规律。方法 C57BL6/J小鼠3只,灌流固定后取肾组织块,树脂812包埋,垂直肾长轴连续半薄切片,从肾被膜到内髓,共得到2 000张2.5μm厚的连续切片,显微镜下获取数字图像,计算机配准,C语言编程,进而追踪26条长髓襻肾单位。结果长髓襻肾单位来自中层和近髓质区的皮质,其髓襻襻曲分布在内髓不同水平,最深的可达肾乳头。通过数字追踪发现,在外髓内带外侧区,髓襻降支细段起始部高度盘曲走行,其盘曲部分的长度是其直线距离的2倍。在盘曲走行中,与同源的髓襻升支粗段间隔排列。最长的长髓襻升支粗段走行在外髓内带的血管丛。在外髓内带的内侧区,髓襻降支细段与髓襻升支粗段紧密相邻,直行进入内髓。结论降支细段在外髓内带高度盘曲走行,在增加了这一节段长度的同时,也增加了这一节段的转运功能,提示此结构特征对该区域的渗透梯度形成可能产生重要影响。
Purpose The formation of medullary osmotic gradient is closely related to the movement and transport function of medulla oblongata. In this study, we plan to establish the medullary law of medulla oblongata. Methods Three C57BL6 / J mice were perfused and fixed. The renal tissue was taken and embedded in the resin 812. The vertical long axis of the kidney was continuously cut into half thin slices. From the kidney capsule to the medulla oblongata, Under the microscope to obtain digital images, computer registration, C language programming, and then track 26 long nephron units. Results The medulla oblongata nephron came from the cortex in the medial and proximal medulla. The medulla oblongata distributed at different levels in the medulla, the deepest reaching the renal papilla. Through digital tracking found that in the lateral medulla oblongata, medulla descending branch of the beginning of a high degree of traverse the curvature of the coil part of the length of its straight line distance of 2 times. In the winding walk, with the same medullary ascending branch thick interval arranged. The longest long medulla oblongata branches walk in the medullary band of vascular plexus. In the medial area of the medullary band, medullary descending branch of the fine segment and the medullary Ascending branch close to the rough, straight into the medulla. Conclusions The descending branch has a high degree of meandering in the outer medulla. While increasing the length of this segment, it also increases the transport function of this segment, suggesting that this structural feature may have a significant impact on the formation of the osmotic gradient in this region .