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目的探讨在携带乙型肝炎病毒的早孕孕妇中,绒毛细胞感染乙型肝炎病毒的发生情况,以及与母婴传播的关系。方法选择自愿在我院门诊行人工流产的孕妇50例,孕妇血清乙型肝炎病毒携带者为实验组(20例),孕妇血清乙型肝炎感染标志物均阴性为对照组(30例)。分别用ELISA法检测外周血血清HBV表面标志物、荧光定量PCR法检测HBV-DNA,用免疫组织化学链霉亲和素-生物素过氧化物酶复合物(SABC)法检测孕妇的绒毛细胞。结果实验组HBV-DNA阳性16例,阴性4例。HbsAg和HbcAg均阳性5例,HbsAg和HbeAg均阳性4例,绒毛细胞出现HBV的阳性染色;对照组HBV-DNA均阴性,HbsAg和HbcAg均阴性,绒毛细胞未出现HBV的阳性染色。结论在早孕人工流产术的乙型肝炎病毒携带孕妇中,乙型肝炎病毒可感染绒毛细胞。
Objective To investigate the incidence of hepatitis B virus infection in villi cells of pregnant women carrying early-onset hepatitis B virus and their relationship with mother-to-infant transmission. Methods Fifty pregnant women who voluntarily induced abortion in our hospital were selected. Pregnant women with hepatitis B virus infection were selected as the experimental group (20 cases). The serum HBV markers were negative in the control group (30 cases). Serum HBV surface markers were detected by ELISA, HBV-DNA by real-time fluorescence quantitative PCR, and the villi cells of pregnant women were detected by immunohistochemical streptavidin-biotin peroxidase complex (SABC). Results In the experimental group, HBV-DNA was positive in 16 cases and negative in 4 cases. HbsAg and HbcAg were positive in 5 cases, HbsAg and HbeAg were positive in 4 cases, villous cells were positive for HBV staining; the control group were negative for HBV-DNA, HbsAg and HbcAg were negative, villus cells did not appear HBV positive staining. Conclusion Hepatitis B virus can infect villus cells in hepatitis B virus-carrying pregnant women with abortion in early pregnancy.