论文部分内容阅读
目的通过对过碘酸钠滴定法和甘油三酯比色法测定解冻红细胞甘油残留量的结果进行比较,探讨应用甘油三酯比色法代替过碘酸钠滴定法进行解冻红细胞甘油残留量测定的可行性。方法配制不同浓度的甘油溶液,比较2种方法的精密度和准确性;测定甘油三酯比色法的线性范围、回收率、干扰试验及其他影响实验因素;用2种方法分别测定30份解冻红细胞标本的甘油残留量,统计分析二者之间的差异。结果甘油三酯比色法测定甘油含量≤2 g/L时有良好的线性关系;过碘酸钠滴定法精密度平均值为6.86,甘油三酯比色法精密度平均值为5.93;过碘酸钠滴定法准确性平均值为112.3%,甘油三酯比色法准确性平均值为97.7%;甘油三酯比色法测定甘油含量回收率平均值为99.5%;测定标本Hb浓度达到12.5 g/L时,对测定结果无干扰(t=1.646,P>0.05);标本血滤液对测定结果无显著影响(t=2.464,P>0.05);血液制剂中甘油三酯含量对测定结果无显著影响;2种方法测定30份解冻红细胞甘油残留量时二者之间差异无统计学意义(t=1.884,P>0.05)。甘油三酯比色法和过碘酸钠滴定法测定甘油残留量时二者之间具统计学意义。结论甘油三酯比色法在测定解冻红细胞甘油残留量时具有方法简单、快速、灵敏度高、稳定性好、回收率高、受干扰因素少等优点,我们认为可以代替过碘酸钠滴定法进行解冻红细胞甘油残留量的质控测定。
OBJECTIVE To compare the results of thawed glycerol glyceride residues by sodium periodate titration and triglyceride colorimetry, and to explore the application of triglyceride colorimetry instead of sodium periodate titration for the determination of residual amount of thawed red blood cell glycerol feasibility. Methods Different concentrations of glycerol solution were prepared and the precision and accuracy of the two methods were compared. The linear range of triglyceride colorimetry, recovery, interference test and other factors affecting the experiment were determined. Thirty samples were thawed Glycerol residue of red blood cell samples, statistical analysis of the difference between the two. Results The triglyceride colorimetry had a good linear relationship when the content of glycerol was less than 2 g / L. The average titration precision of sodium periodate was 6.86, and the precision of triglyceride colorimetry was 5.93. The average accuracy of sodium acid titration was 112.3%, the accuracy of triglyceride colorimetric method was 97.7%, the average recovery of glycerol was 99.5% by triglyceride colorimetry, and the concentration of Hb was 12.5 g / L, there was no interference with the determination results (t = 1.646, P> 0.05). The blood filtrate of the sample had no significant effect on the determination results (t = 2.464, P> 0.05) There was no significant difference between the two methods in the determination of glycerol residues in 30 thawed erythrocytes (t = 1.884, P> 0.05). Triglyceride colorimetric method and sodium periodate titration method for the determination of residual glycerol between the two statistically significant. Conclusion Triglyceride colorimetric assay has the advantages of simple, rapid, high sensitivity, good stability, high recovery, less interference factors and so on, which can be used in place of sodium periodate titration Quality Control Measurement of Thawed Red Cell Glycerol Residues.