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目的探讨1型糖尿病小鼠肝脏线粒体功能的变化。方法将10只6周龄C57BL/6J雄性小鼠随机分为两组:1腹腔注射链脲霉素造成1型糖尿病小鼠模型;2正常对照。实验期间监测血糖、体重等指标;造模结束后,用腹腔注射葡萄糖耐量实验(IPGTT)和胰岛素耐量实验(ITT)评估糖代谢,提取肝脏线粒体做氧化磷酸化测定,同时检测线粒体膜极性、钙离子蓄积能力、过氧化氢产生、柠檬酸合酶活性;检测血清和肝组织活性氧、总超氧化物歧化酶、丙二醛和总抗氧化能力等。结果与对照小鼠相比,1型糖尿病小鼠在高血糖的同时胰岛素敏感度亦显著上调,肝脏线粒体分别加入复合物Ⅰ、复合物Ⅱ和复合物Ⅳ的底物后耗氧率明显上升(P<0.05);肝脏活性氧(P<0.01)和丙二醛(P<0.05)含量明显上升,总抗氧化能力明显下降(P<0.05)。结论 1型糖尿病小鼠肝脏线粒体电子传递链氧化磷酸化的活性明显增强,可能导致氧化应激的发生。
Objective To investigate the changes of hepatic mitochondrial function in type 1 diabetic mice. Methods Ten 6-week-old C57BL / 6J male mice were randomly divided into two groups: 1 mice were injected intraperitoneally with streptozotocin to induce type 1 diabetic mice; 2 normal control. Glucose metabolism was assessed by intraperitoneal injection of glucose tolerance test (IPGTT) and insulin tolerance test (ITT), the mitochondria were extracted for oxidative phosphorylation assay, and the mitochondrial membrane polarity, Calcium ion accumulation capacity, hydrogen peroxide production, citrate synthase activity; detection of serum and liver reactive oxygen species, total superoxide dismutase, malondialdehyde and total antioxidant capacity. Results Compared with the control mice, the type 1 diabetic mice also had significantly increased insulin sensitivity in hyperglycemia. The oxygen consumption rate of liver mitochondria after addition of complex Ⅰ, complex Ⅱ and complex Ⅳ was significantly increased P <0.05). The levels of reactive oxygen species (P <0.01) and malondialdehyde (P <0.05) in liver increased significantly and the total antioxidant capacity decreased significantly (P <0.05). Conclusion The oxidative phosphorylation activity of mitochondrial electron transport chain in liver of type 1 diabetic mice is significantly increased, which may lead to the occurrence of oxidative stress.