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目的:建立同时测定多花蒿药材中6种绿原酸类成分的方法.方法:采用HPLC法,Kromasil 100-5 C18色谱柱(150 mm×4.6 mm,5 μm),流动相为0.1％乙酸水溶液(A)-乙腈(B),采用梯度洗脱,流速为1.0 mL·min-1,检测波长为327 nm,柱温为25 ℃.结果:50 min内新绿原酸(3-CQA)、绿原酸(5-CQA)、隐绿原酸(4-CQA)、3,4-二咖啡酰奎宁酸(3,4-DiCQA)、3,5-二咖啡酰奎宁酸(3,5-DiCQA)和4,5-二咖啡酰奎宁酸(4,5-DiCQA)6个绿原酸类成分分离度良好,且在各自的检测范围内与峰面积呈现良好的线性关系,其相关系数均＞0.9997,加样回收率为98.06％～98.96％.应用所建立的方法对不同来源的多花蒿药材中的绿原酸类成分进行了含量测定.结论:所建立的含测方法准确可靠,可为多花蒿药材的质量控制提供参考.“,”Objective: To establish an HPLC-DAD method for simultaneous determination of 6 types of chlorogenic acids in Artemisia myriantha. Methods: The separation was achieved on a Kromasil 100-5 C18 analytical column (150 mm×4.6 mm, 5 μm) with a gradient elution of 0.1％ acetic acid (A) and acetonitrile (B) at a flow rate of 1.0 mL·min-1. The detection wavelength was 327 nm and the column temperature was 25 ℃. Results: Perfect chromatogram with good separation of 6 phenolic acids including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 4,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid and 3,5-dicaffeoylquinic acid was obtained within 50 minutes. All the calibration curves showed good linearity between concentration of each compound and peak area. And the values of r were no less than 0.999,7 for all the analytes. The recoveries were 98.06％-98.96％. The developed method was successfully applied for the analysis of 6 chlorogenic acids in Artemisia myriantha obtained from 6 different provinces. Conclusion: The developed method is accurate and reliable, and can be helpful for determination of chlorogenic acids and quality control of Artemisia myriantha.