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本实验动态测定了体外细胞培养过程中兔肺泡巨噬细胞存活率和超氧阴离子自由基，探讨了乙二胺四乙酸（ＥＤＴＡ）对桦木尘毒作用的影响。结果表明，经ＥＤＴＡ处理的桦木尘与未经处理的桦木尘相比较，前者在各接尘时段（６、１２、１８、２４小时）诱发巨噬细胞产生Ｏ－·２（超氧阴离子的自由基）的含量显著下降，在１２、１８小时两时段巨噬细胞存活率显著升高。这提示，适当浓度ＥＤ－ＴＡ在一定作用时间范围内可部分阻断桦木尘诱发巨噬细胞产生Ｏ－·２，对巨噬细胞起到保护作用。
This experiment dynamically measured in vitro cell culture of rabbit alveolar macrophage survival rate and superoxide anion free radicals, ethylenediaminetetraacetic acid (EDTA) on the impact of birch dust. The results showed that compared with the untreated birch dust, the EDTA-treated birch dust induced the macrophages to produce O- · 2 (superoxide anion free) at each dusting period (6, 12, 18, 24 hours) ) Significantly decreased the content of macrophages at 12 and 18 hours. This suggests that the appropriate concentration of ED-TA can partially block the birch dust-induced generation of O · 2 in macrophages within a certain action time range, which plays a protective role on macrophages.