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在内源性激素存在的条件下,用交换法测定了大鼠肝胞液的糖皮质激素受体。交换条件为Oc4.5～5小时。在这条件下,[~3H]皮质酮—受体复合物的解离将近90％。向去肾上腺大鼠肝胞内预先加入不同量的皮质酮保温后,再测定[~3H]地塞米松特异结合,其结果表明,胞液皮质酮浓度在6nm以内时,[~3H]地塞米松特异结合容量(Ro)的测定值的减少不超过10％,表面上的解离常数(Kd)的测定值基本上不变。用这方法测定烫伤大鼠肝胞液的Ro和Kd,结果Ro减少。烫伤后1、12、24、36小时和5天的Ro分别为对照组的67.4±15.2、70.6±22.0、79.7±5.8、113.0±24.8和102.9±17.6％(均值±标准差)。烫后1小时Kd增大。对其可能的机理作了讨论。
In the presence of endogenous hormones, the glucocorticoid receptor in rat liver cytosol was determined by the exchange method. The exchange condition is Oc4.5 ~ 5 hours. Under this condition, the [~ 3H] corticosterone-receptor complex dissociates by nearly 90%. To adrenal rat liver intracellular pretreatment with different amounts of corticosterone, and then measured [~ 3H] dexamethasone specific binding, the results showed that the cytosolic cortexone concentrations within 6nm, [~ 3H] to plug The decrease in the measured value of the specific binding capacity (Ro) of the mussel does not exceed 10%, and the measured value of the dissociation constant (Kd) on the surface is substantially constant. This method was used to determine Ro and Kd in liver cells from scalded rats, resulting in a decrease in Ro. Ro at 1, 12, 24, 36 and 5 days after scald were 67.4 ± 15.2, 70.6 ± 22.0, 79.7 ± 5.8, 113.0 ± 24.8 and 102.9 ± 17.6%, respectively, of the control group (mean ± standard deviation). 1 hour after hot Kd increased. The possible mechanism is discussed.