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目的研究组织蛋白酶Ｄ（Ｃａｔｈ┐Ｄ）对乳腺癌侵袭、转移和预后的作用。方法采用免疫组织化学亲和素┐生物素┐过氧化物酶复合物（ＳＡＢＣ）法，检测１０５例乳腺癌石蜡切片中肿瘤细胞（实质）和间质细胞组织蛋白酶Ｄ的表达，结合临床和病理资料分析其作用。结果组织蛋白酶Ｄ主要定位于细胞浆，部分增生的细胞可见定位于细胞核，间质细胞与实质细胞的Ｃａｔｈ┐Ｄ的定位基本相似。间质细胞以肿瘤周围的间质细胞组织蛋白酶Ｄ的表达量为高。Ｃａｔｈ┐Ｄ在乳腺癌实质和间质中总的表达率分别为４９．５％（５２／１０５），５６．２％（５９／１０５）。实质Ｃａｔｈ┐Ｄ的表达与肿瘤细胞组织浸润、肿瘤大小、腋淋巴结转移、随访结果有关（Ｐ＜０．０５）；与乳腺癌组织学分类、组织学分型、分级、年龄、ＥＲ、ＰＲ状态、局部复发、远处转移无关。间质Ｃａｔｈ┐Ｄ的表达与乳腺癌肿瘤大小、腋淋巴结转移、局部复发、远处转移、年龄、ＥＲ、ＰＲ状态、局部复发无关。实质与间质Ｃａｔｈ┐Ｄ的表达和乳腺癌患者的无瘤生存率有关（Ｐ＜０．０５）；实质Ｃａｔｈ┐Ｄ表达与总生存率无关（Ｐ＞０．０５），而间质的Ｃａｔｈ┐Ｄ与总生存率有关（Ｐ＜０．０５）。结论实质和间质乳腺癌组织蛋白酶Ｄ的表达都和?
Objective To study the effect of cathepsin D (CathD) on invasion, metastasis and prognosis of breast cancer. Methods The immunohistochemical avidin-biotinylated peroxidase complex (SABC) method was used to detect the expression of cathepsin D in tumor cells (parenchyma) and mesenchymal cells in paraffin sections from 105 breast cancer patients. Data analysis of its role. Results Cathepsin D was mainly localized in the cytoplasm, and partially proliferated cells were localized in the nucleus. The localization of Cath D in the stromal cells and parenchymal cells was similar. In stromal cells, the expression of cathepsin D in the stromal cells around the tumor was high. The total expression rate of Cath┐D in the parenchyma and stroma of breast cancer was 49.5% (52/105) and 56.2% (59/105), respectively. The expression of parenchyma Cath D was related to tumor tissue infiltration, tumor size, axillary lymph node metastasis, follow-up results (P<0.05); and histological classification, histological type, grade, age, ER, PR status, Local recurrence, distant metastasis has nothing to do. The expression of Cathth D in the interstitium was not associated with breast cancer tumor size, axillary lymph node metastasis, local recurrence, distant metastasis, age, ER, PR status, and local recurrence. The parenchyma was related to the expression of Cathepsin D and the tumor-free survival rate of breast cancer patients (P<0.05); the expression of Cathth D was not related to the overall survival (P>0.05), while the interstitial Cath ┐D was associated with overall survival (P<0.05). Conclusion The expression of cathepsin D in both parenchymal and interstitial breast cancers are both consistent.