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目的研究内蒙古地区炭疽芽胞杆菌基因单核苷酸多态性(SNP)特征。方法选择内蒙古地区不同分离年代、地点和来源的22株炭疽芽胞杆菌,采用实时荧光定量聚合酶链反应(real-time PCR)方法扩增染色体上的13个SNP位点,进行聚类分析。结果使用的13个SNP位点中8个位点相同,5个位点存在多态性。通过聚类分析,22株炭疽芽胞杆菌可分为4个群,属于A.Br.Aust94亚群和A.Br.008/009亚群的菌株各有1株,A.Br.Ames亚群和A.Br.001/002亚群菌株占大多数。结论内蒙古地区炭疽芽胞杆菌基因SNP位点具有遗传稳定性,目前5个SNP位点可作为内蒙古地区炭疽杆菌荚膜质粒SNP位点基因分型的指标,A.Br.Ames亚群和A.Br.001/002亚群是内蒙古地区优势亚群。
Objective To study the characteristics of single nucleotide polymorphism (SNP) of Bacillus anthracis in Inner Mongolia. Methods 22 strains of B. anthracis from different ages, locations and sources in Inner Mongolia were selected and 13 SNPs on chromosomes were amplified by real-time PCR and clustered. As a result, 8 of the 13 SNP loci were the same, and 5 loci were polymorphic. By cluster analysis, 22 strains of Bacillus anthracis could be divided into four groups, one strain belonging to A.Br.Aust94 subgroup and A.Br.008 / 009 subgroup, one strain of A.Br.Ames subgroup and A.Br.001 / 002 sub-group of strains accounted for the majority. Conclusion The SNP loci of Bacillus anthracis in Inner Mongolia have genetic stability. At present, five SNP loci can be used as SNP loci markers for Bacillus anthracis capsici in Inner Mongolia. A.Br.Ames subpopulations and A.Br .001 / 002 sub-group is the dominant sub-group in Inner Mongolia.