Imprinted Human Serum Albumin with Antioxidant Activity

• 作 者: SHEN Na YAN Fei GUO Yi L Shao-
• 机 构: SHEN Na,Key Laboratory of Molecular Enzymology and Engineering,State Key Laboratory of Industrial Co
• 出 处: 高等学校化学研究（英文版）
• 发表日期: 2004年期
• 关键词: Antioxidant Enzyme mimic Glutathione peroxidase Molecular imprinting

In order to create a new mimic of glutathione peroxidase(GPx), bioimprinting was used to generate glutathione(GSH) binding site and chemical modification was used to incorporate catalytic group selenocystine(Sec).Human serum aibumin(HSA) and S-substituted dinitrophenyl glutathione(GSH-S-DNP) were chosen as the imprinted matrix and imprinting template, respectively, to generate a GSH-imprinted protein(GSH-HSA) by bioimprinting. Sec was incorporated into the GSH-HSA by chemical modification to give a new GPx mimic(Se-GSH-HSA).Se-GSH-HSA displayed considerably higher GPx activity than non-printed HSA(Se-HSA). The enzymic properties and kinetics of Se-GSH-HSA were studied. Moreover, Se-GSH-HSA was confirmed to have stronger antioxidant ability to protect mitochondria against oxidative damage with ferrous sulfate/ascorbate-induced mitochondria damage model, indicating that Se-GSH-HSA has potential application in medicine.