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目的 探讨nm23基因在人肺癌发生、发展中的作用。方法 采用狭缝印迹杂交技术和非放射性地高辛标记检测系统,检测了143份不同部位、不同性质人肺组织中的nm23H1和nm23H2基因的mRNA表达。结果 nm23H1和nm23H2基因的mRNA表达均有从正常肺组织、肺良性病变组织、非癌肺组织、癌灶组织到癌转移淋巴结组织逐渐降低的趋势。其中,肺癌组织的nm23H2基因mRNA表达较正常肺组织显著较低(P<0.05),癌转移淋巴结组织的nm23H1和nm23H2基因mRNA表达均较正常肺组织显著降低(P<0.05)。肺癌组织的nm23基因表达与淋巴结有无癌转移无明显关系(P>0.05)。结论 nm23基因表达降低可能与肺癌发生有关,未发现nm23基因有癌转移抑制作用
Objective To investigate the role of nm23 gene in the development and progression of human lung cancer. Methods The mRNA expression of nm23H1 and nm23H2 genes in 143 human lung tissues with different sites and different characteristics were detected by slot blot hybridization and non-radioactive digoxin labeling system. Results The mRNA expressions of nm23H1 and nm23H2 genes were gradually decreased from normal lung tissue, benign lesion tissue, non-cancer lung tissue, tumor tissue to cancer metastatic lymph node tissue. Among them, the expression of nm23H2 mRNA in lung cancer tissues was significantly lower than that in normal lung tissues (P<0.05). The nm23H1 and nm23H2 gene mRNA expressions in cancer metastasis lymph node tissues were significantly lower than those in normal lung tissues (P<0.05). The expression of nm23 in lung cancer tissues was not significantly related to lymph node metastasis (P>0.05). Conclusion The decrease of nm23 gene expression may be related to the occurrence of lung cancer. No nm23 gene is found to have metastasis suppressive effect.