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目的探索男性非淋菌性尿道炎患者尿道黏膜细胞内衣原体始体与包涵体检测的意义。方法取患者尿道粘膜柱状上皮细胞涂片姬姆萨染色镜检。结果男性非淋菌性尿道炎患者尿道黏膜细胞内广泛存在衣原体始体与包涵体,在衣原体敏感抗生素治疗前后衣原体感染细胞百分比差异有统计学意义(P<0.01),尿道细胞标本经实时荧光定量聚合酶链反应检测含有衣原体DNA,经免疫组化染色检测含有衣原体抗原抗体免疫复合物。结论提示衣原体是男性非淋菌性尿道炎患者的主要致病菌,尿道黏膜细胞内存在衣原体包涵体与数个始体是衣原体感染的确凿证据。
Objective To explore the significance of detecting the bodies and inclusion bodies of C. pylori in urethral mucosa of male patients with non-gonococcal urethritis. Methods The urethral mucosa columnar epithelial cells smear Giemsa staining. Results There were widespread chlamydial and inclusion bodies in the urethral mucosal cells of male patients with non-gonococcal urethritis. The percentage of chlamydia-infected cells was significantly different before and after treatment with chlamydia-sensitive antibiotics (P <0.01). The urethral cells were quantified by real- Enzyme-linked immunosorbent assay (PCR) detection of chlamydial DNA, immunohistochemical detection of chlamydial antigen-containing antibody immune complexes. Conclusions Chlamydia is the main causative agent of male patients with non-gonococcal urethritis, urethral mucosal cells in the presence of chlamydial inclusion bodies and several starters are conclusive evidence of chlamydial infection.