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植物种子从休眠到萌发的转变是其生命周期中一个关键的生理过程,其中,植物生长调节剂发挥了重要的调控作用,而SDR1(short-chain dehydrogenase/reductase 1)是调控营养信号转导和植物生长调节剂合成的关键酶。本研究克隆获得了福恢7185、福恢653和云恢290中SDR1基因的CDS序列,比对发现其在3个不同休眠品种并无差异,但与NCBI上日本晴的基因序列相比,缺失了9个碱基,3个丙氨酸;结合qRT-PCR技术分析SDR1基因在不同休眠水稻品种种胚中的表达情况,探索其与种子休眠的关系,同时利用GST pull-down技术找到SDR1的1个互作蛋白,结果表明,该蛋白与休眠相关,初步表明SDR1基因可能通过与其它结构原件的互作来调控种子休眠。
Plant seed transition from dormancy to germination is a key physiological process in its life cycle, in which plant growth regulators play an important regulatory role, and SDR1 (short-chain dehydrogenase / reductase 1) is a regulator of vegetative signal transduction and Plant growth regulator synthesis of key enzymes. In this study, the CDS sequences of SDR1 gene in Fuhui 7185, Fuhui 653 and Yunhui 290 were cloned. The sequence comparison showed that there was no difference in the three different dormancy cultivars, however, compared with the sequence of Nipponbare on NCBI, 9 bases and 3 alanines. The expression of SDR1 gene in embryos of different dormant rice varieties was analyzed by qRT-PCR and the relationship between SDR1 gene and seed dormancy was explored. At the same time, 1 The results showed that the protein was related to dormancy, suggesting that SDR1 may regulate seed dormancy through interaction with other structural elements.