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最近,不少文章介绍微分吸光光度法可以大大提高吸光光度法的灵敏度,石井一指出模拟微分吸光光度法的灵敏度是普通吸光光度法的4.5~n倍,n为微分阶次。吴家齐等也讨论了数值计算法的微分吸光光度法的灵敏度问题。但是,我们认为,灵敏度是否被提高,不能只看仪器对被测物产生多大的信号,同时还应该考查噪声水平。即应看微分前后的信噪比是否得到改善。为此,我们系统地研究了普通吸光光度法、二阶模拟微分法以及日立557的四阶数值微分法对于:
Recently, many articles introduce differential absorbance spectrophotometry can greatly enhance the sensitivity of absorbance spectrophotometry, Ishii pointed out that the sensitivity of analog differential spectrophotometry is 4.5 ~ n times the ordinary absorbance spectrophotometry, n is the order of differentiation. Wu et al also discussed the numerical method of differential sensitivity spectrophotometry sensitivity problems. However, in our opinion, the sensitivity has not been raised and we can not just look at how much the instrument generates signals to the measured object, but also check the noise level. That is to say whether the signal-to-noise ratio before and after differentiation is improved. To this end, we systematically studied the ordinary photometry, second-order analog differential method and Hitachi 557 fourth-order numerical differential method for: