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目的 观察白藜芦醇对非酒精性脂肪肝的治疗效果,并初步探讨其机制. 方法 Wistar大鼠3组(每组10只):正常对照(NC)组以标准饲料喂养,高脂饲料喂养(HF)组和白藜芦醇治疗(RT)组以高脂饲料喂养,RT组第7周开始加用白藜芦醇治疗(100mg·kg-1·d).在第16周结束实验,以正糖-高胰岛素钳夹术评价大鼠胰岛素抵抗;肝脏石蜡切片HE染色观察病理学改变;Western blot技术检测肝脏内5’-磷酸腺苷活化白激酶(AMPK)的磷酸化水平. 结果 HF组与NC组相比,内脏脂肪和肝指数增高(P<0.05);空腹胰岛素水平增高,葡萄糖输注率下降(t=10.554,P<0.01);光学显微镜下肝脏出现明显脂肪变性;肝组织内AMPK磷酸化水平降低到NC组的45.8%(t=8.384,P<0.01).白藜芦醇干预后,内脏脂肪指数显著下降(P<0.01);空腹胰岛素水平降低,葡萄糖输注率明显增高(t=5.866,P<0.01);光学显微镜下的肝脏脂肪变性得到明显改善;肝组织内的AMPK磷酸化水平提高到NC组的70.2%(t=3.816,P<0.01).结论 白藜芦醇通过AMPK途径可以同时改善胰岛素抵抗和减少肝脏脂质沉积,从而改善脂肪肝.“,”Objective To study the effect of resveratrol on the nonalcoholic fatty liver (NAFL). Method Thirty Wistar rats were allocated into three groups (10 for each): a normal control group (NC, fed with standard chow), a high fat feeding group (HF, fed with a high fat diet) and a resveratrol treated group (RT). The RT rats were fed a high fat diet all the time (as the HF) and received resveratrol administration rats were sacrificed and hyperinsulinemic-euglycemic clamp was performed to evaluate insulin sensitivity. Liver histology was studied by HE stained microscopic preparations of the livers. Phosphorylation of AMP- activated protein ldnase (AMPK) levels were determined by Western blot. Results Compared to the NC, visceral fat index and liver mass index was lower and fasting serum insulin (FINS) was higher and glucose infusion rate (GIR) was lower in the HF (t = 10.554, P < 0.01). Severe liver steatosis was observed micro- scopically in the HF. In addition, phosphorylation of AMPK levels in the HF was 45.8% lower than that of the NC (t = 8.384, P < 0.01). Compared with the HF, the visceral fat index, liver mass index, FINS and GIR were all much lower, and the liver steatosis was milder than those in the liT. Further phosphorylation of AMPK seen was 70.2% higher in RT compared with that in the NC (t = 3.816, P < 0.01). Conclusion Resveratrol administration improved high-fat feeding induced insulin resistance and fatty liver by activating AMPK.