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AIM:To investigate the expression of p27,cyclin E andcyclin A in hepatocellular carcinoma (HCC) and its potentialclinical significance.METHODS:Expression of p27,cyclin E and cyclin A in 45HCC specimens and 30 adjacent noncancerous lesionsobtained from 45 patients during surgery was examined byimmunohistochemical SABC assay.The diameter of tumorranged from 1 cm to 19 cm (d≤5 cm,9 samples;5 cm10 cm,17 samples).The tumors weregraded according to the criteria described by Edmondson-Steiner:well-differentiated HCC group (Grade Ⅰ+Ⅱ),26samples;poorly-differentiated HCC group (Grade Ⅲ+Ⅳ),19 samples.According to the clinical-pathologic features,19 samples were poorly encapsulated,15 samples had portalinvasion of cancer,11 samples had extrahepatic metastasis,and 12 samples had intrahepatic metastasis.All of thesamples were classified as the invasive and metastatic group,while the remaining was classified as the non-invasive andnon- metastatic group.RESULTS:The average labeling index (LI) of p27 in HCClesions was significantly higher than that in adjacentnoncancerous lesions (45.87±14.21 vs33.77±12.92,t=3.745,P<0.001).The LI of p27 was associated with differentiation,invasiveness and metastasis of the tumors (34.46±12.29 vs52.80±11.36,t=5.17;41.42±12.86 vs51.44±14.10,t=2.48;P<0.05).Cyclin E was overexpressed in 16 cases (35.6%)while cyclin A was overexpressed in 21 cases (46.7%) inHCC lesions.No overexpression of cyclin E or cyclin A couldbe observed in adjacent non-carcinoma lesions and normalliver tissues.The overexpressions of cyclin E and cyclin Awere correlated with differentiation,tumor thrombus,invasiveness and metastasis (P<0.05).Expression of cyclinE was significantly correlated with expression of cyclin A(r=0.329,P<0.05).The LI of p27 was significantlydecreased in cyclin E,cyclin A positive groups (40.33±11.91vs 49.50±13.76,t=3.05;38.86±11.19 vs 52.57±12.62,t=3.89;P<0.05).CONCLUSION:p27,cyclin E,cyclin A play cooperative rolesin HCC tumorigenesis,differentiation,invasiveness andmetastasis.Detection of their expression may be helpful inprediction of tumor progression.
AIM: To investigate the expression of p27, cyclin E and cyclin A in hepatocellular carcinoma (HCC) and its potential clinical significance. METHODS: Expression of p27, cyclin E and cyclin A in 45HCC specimens and 30 adjacent noncancerous lesion bound from 45 patients during surgical was surgery byimmunohistochemical SABC assay. The diameter of tumorranged from 1 cm to 19 cm (d ≦ 5 cm, 9 samples; 5 cm 10 cm, 17 samples). described by Edmondson-Steiner: well-differentiated HCC group (Grade I + II), 26 samples; poorly-differentiated HCC group (Grade III + IV), 19 samples. According to the clinical-pathologic features, 19 samples were poorly encapsulated, 15 samples had portal invasion of cancer, 11 samples had extrahepatic metastasis, and 12 samples had intrahepatic metastasis. All of the samples were classified as the invasive and metastatic group, while the remaining was classified as the non-invasive and non-metastatic groups .RESULTS: The a verage labeling index (LI) of p27 in HCClesions was significantly higher than that of adjacent noncancerous lesions (45.87 ± 14.21 vs33.77 ± 12.92, t = 3.745, P <0.001). The LI of p27 was associated with differentiation, invasiveness and metastasis of the tumors (34.46 ± 12.29 vs.52.80 ± 11.36, t = 5.17; 41.42 ± 12.86 vs51.44 ± 14.10, t = 2.48, P <0.05) .Cyclin E was overexpressed in 16 cases (35.6%) while cyclin A was overexpressed in 21 cases (46.7%) of inHCC lesions. No overexpression of cyclin E or cyclin A could be observed in adjacent non-carcinoma lesions and normalliver tissues. The overexpressions of cyclin E and cyclin Awere correlated with differentiation, tumor thrombus, invasiveness and metastasis (P <0.05) .Expression of cyclinE was significantly correlated with the expression of cyclin A (r = 0.329, P <0.05). The LI of p27 was significantlycreased in cyclin E, cyclin A positive groups (40.33 ± 11.91 vs 49.50 ± 13.76, t = 3.05; 38.86 ± 11.19 vs 52.57 ± 12.62, t = 3.89; P <0.05) .CONCLUSION: p27, cyclin E, cyclin A play cooperative ro lesin HCC tumorigenesis, differentiation, invasiveness andmetastasis. Detection of their expression may be helpful inprediction of tumor progression.