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目的：建立一种确实可行的诱导肾移植免疫耐受的动物模型，以探讨肾移植后的免疫耐受情况。方法：采用供体ＳＤ大鼠脾细胞，经过处理后注入受体Ｗｉｓｔａｒ大鼠的门静脉内，同时进行肾移植，然后用环孢素治疗一周的方法。结果：实验组大鼠平均成活时间为７２２±３２天，对照组为１０５±２１天，实验组与对照组比较Ｐ＜００１。实验组在肾移植６０天后，再用ＳＤ大鼠和Ｌｅｗｉｓ大鼠的皮肤进行移植，发现ＳＤ大鼠的皮肤不被排斥，Ｌｅｗｉｓ大鼠的皮肤出现排斥。结论：门静脉内注射供体脾细胞可诱导肾移植免疫耐受，并且这种耐受具有特异性。
OBJECTIVE: To establish a viable animal model for inducing immune tolerance in renal transplantation in order to investigate the immune tolerance after renal transplantation. METHODS: Splenocytes from donor Sprague-Dawley rats were injected into the portal vein of recipient Wistar rats, and at the same time, the kidney was transplanted and then treated with cyclosporine for one week. Results: The mean survival time of rats in experimental group was 722 ± 32 days and that of control group was 105 ± 21 days, P <001 in experimental group and control group. The experimental group was transplanted on the skin of SD rats and Lewis rats 60 days after kidney transplantation. The skin of SD rats was not rejected and the skin of Lewis rats was rejected. Conclusions: Intravenous injection of donor splenocytes induces immune tolerance in renal transplant recipients, and this tolerance is specific.