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Objective:To investigate whether the cell adhesion molecule integrin αV could influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells via effecting intercellular effect.Methods:Human NPC cell line CNE-2Z was cultivated as three dimensional model using liquid overlay technique.Cell counting was employed to detect the radiosensitivity of CNE-2Z cells by blood cell counter before and after blocking integrin αV function;cell apoptosis was detected by flow cy-tometry using Annexin V/PI label.Results:Three dimensional culture model of human NPC cell line CNE-2Z cells were suc-cessfully established.The integrin αV expression of CNE-2Z multicellular spheroids elevated as the dosage of radiotherapy increased.Blocking of integrin αV function leaded to higher radiosensitivity and more apoptotic cells of CNE-2Z multicellular spheroids.Conclusion:Cell adhesion molecules integrin αV can influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells and the inhibition of cell apoptosis might be its mechanism,proved by three dimensional culture model to mimic solid tumor in vivo.
Objective: To investigate whether the cell adhesion molecule integrin αV could influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells by effecting intercellular effect. Methods: Human NPC cell line CNE-2Z was cultivated as three dimensional model using liquid overlay technique. Cell counting was employed to detect the radiosensitivity of CNE-2Z cells by blood cell counter before and after blocking integrin alpha V function; cell apoptosis was detected by flow cy-tometry using Annexin V / PI label. Results: Three dimensional culture model of human NPC cell line CNE-2Z cells were suc-cessfully established. The integrin α V expression of CNE-2Z multicellular spheroids elevated as the dosage of radiotherapy increased. Blocking of integrin α V function leaded to higher radiosensitivity and more apoptotic cells of CNE-2Z multicellular spheroids. Conlusion: Cell adhesion molecule integrin αV can influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells and the i nhibition of cell apoptosis might be its mechanism, proven by three dimensional culture model to mimic solid tumor in vivo.