论文部分内容阅读
采用L9(34)正交设计将戊己丸中黄连,吴茱萸,白芍提取物组成9组配伍方,以及相应剂量的单味药方,共计18个方。建立并采用UPLC-MS/MS方法测定并比较不同配伍方代表成分小檗碱、巴马汀、吴茱萸碱、吴茱萸次碱、芍药苷在大鼠口服给药2 h后在肝脏中的浓度比较戊己丸不同配伍方中有效成分在大鼠肝脏中的浓度。结果显示戊己丸配伍方中有效成分在肝中的浓度与相同剂量的单味药组方存在明显差异,相同剂量配伍方之间也存在差异。黄连与低、高剂量吴茱萸中吴茱萸碱浓度成正相关,吴茱萸与低剂量黄连中小檗碱的浓度成负相关,与中剂量黄连中小檗碱的浓度成正相关,与中剂量白芍中芍药苷浓度成正相关。白芍与中剂量黄连中巴马汀浓度成负相关、与中剂量吴茱萸中吴茱萸碱、吴茱萸次碱浓度成负相关。提示了黄连、吴茱萸、白芍之间相互作用导致了各活性成分在肝中分布的差异,分析显示各代表成分2 h在肝中浓度最大的配伍比为12∶6∶6。
Orthogonal design of L9 (34) will be Wuji pill Coptis Chinensis, Evodia, Radix Paeoniae Alba constitute 9 groups of prescriptions, and the corresponding dose of single prescription, a total of 18 parties. The concentrations of berberine, palmatine, evodiamine, rutaecarpine and paeoniflorin in rats were determined and compared by UPLC-MS / MS method in rats after oral administration for 2 h. Concentration of active ingredients in different compatibility prescriptions in rat liver in rat liver. The results showed that there was a significant difference between the concentration of active ingredient in the liver of Wuji Pills and the single dose of the same dose of prescription, there were also differences between the same dose and compatibility prescription. Coptis and low and high doses of evodiamine Evodiamine concentration is positively related to Evodia and low doses of berberine in the concentration of a negative correlation between concentration and berberine in the dose of berberine concentration is positively correlated with the dose of paeoniflorin positive Related. Radix Paeoniae Alba and medium dose of palmatine in the concentration of a negative correlation, and the dose of evodiamine evodiamine, rutaecarpine concentration negatively correlated. Prompted Coptis, Evodia, peony root interaction led to the distribution of the active ingredient in the liver, the analysis showed that the representative components 2 h in the liver maximum concentration ratio of 12:6:6.