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南方水稻黑条矮缩病毒(Southern rice black-streaked dwarf virus,SRBSDV)是呼肠孤病毒科(Reoviridae)斐济病毒属(Fijivirus)成员,主要由白背飞虱(Sogatella furcifera,WBPH)以持久增殖型方式进行传播。在病毒的侵染循回过程中,白背飞虱中肠上皮细胞是病毒的初侵染和主要增殖场所。而病毒的有效增殖是决定介体能否传毒的关键影响因素。为了更好地研究SRBSDV和介体白背飞虱的互作关系,尤其是了解白背飞虱中肠蛋白通过参与调控病毒的增殖过程,而使介体昆虫成功获毒并传毒,本研究以高带毒白背飞虱群体中肠组织为实验材料,构建了高带毒白背飞虱群体中肠的酵母双杂交c DNA文库。经过检测表明,构建的文库滴度为1.5×106cfu/m L,平均插入片段主要分布在1.0~2.0 kb之间,文库质量较好,可用于研究SRBSDV编码蛋白和白背飞虱中肠蛋白的互作关系,并为开展SRBSDV和昆虫介体的互作研究奠定了基础。
Southern rice black-streaked dwarf virus (SRBSDV) is a member of the Fijivirus family of Reoviridae and is mainly composed of Sogatella furcifera (WBPH) Type of way to spread. During the process of virus infection, the intestinal epithelial cells of white-backed planthopper are the primary infection and major proliferating site of the virus. The effective proliferation of the virus is the key factor that determines whether the mediator can be transmitted. In order to better study the interaction between SRBSDV and the white-backed planthopper, Siphonopsis pensile, especially understand the intestine protein of white-backed planthopper by mediating the proliferation of the virus, Taking the midgut tissue of high-belt-poisoning white-backed planthopper populations as experimental materials, a yeast two-hybrid cDNA library was constructed. The results showed that the titer of constructed library was 1.5 × 106cfu / m L, the average inserted fragment was mainly distributed between 1.0-2.0 kb, and the library was of good quality and could be used to study the expression of SRBSDV protein And laid the foundation for the research on the interaction between SRBSDV and insect mediators.