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目的采用RP-HPLC法同时测定腰痛宁贴片中士的宁和马钱子碱的含量。方法采用Inertsil C8色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-冰醋酸(100∶480∶35,三乙胺调pH3.2),流速1.0 mL.min-1,检测波长254 nm,柱温30℃,进样量10μL。结果士的宁和马钱子碱的线性范围分别为2.0～10.0μg(r=0.9998)、1.8～9.0μg(r=0.9997),平均回收率分别为98.9%(RSD=1.19%)、100.7%(RSD=1.49%),3批腰痛宁贴片中士的宁和马钱子碱的平均含量分别为5.15、4.03 mg。结论所建方法准确可靠,可用于腰痛宁贴片中士的宁和马钱子碱的含量测定。
Objective To determine the contents of strychnine and brucine in Yaotongning patch by RP-HPLC. Methods Inertsil C8 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was methanol-water-glacial acetic acid (100: 480: 35, triethylamine adjusted to pH3.2) and the flow rate was 1.0 mL.min-1. Wavelength 254 nm, column temperature 30 ℃, injection volume 10μL. Results The linear ranges of strychnine and brucine were 2.0 ~ 10.0μg (r = 0.9998) and 1.8 ~ 9.0μg (r = 0.9997) respectively. The average recoveries were 98.9% (RSD = 1.19%) and 100.7% (RSD = 1.49%). The average contents of strychnine and brucine in three batches of Yaotongning patch were 5.15 and 4.03 mg, respectively. Conclusion The method is accurate and reliable and can be used for the determination of strychnine and brucine in Yaotongning patch.