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目的探讨B7-H4、P53、CEA在胃癌中的表达特点及临床意义,寻找胃癌早期诊断的免疫学指标。方法用免疫组织化学方法测定B7-H4、P53、CEA在正常胃黏膜、癌前疾病、胃癌中的表达情况。结果 B7-H4在胃癌中高表达,其表达强度与浸润深度、淋巴结转移相关。生存期≤5年的患者B7-H4阳性表达率高于生存期>5年的患者,差异有统计学意义(P<0.05)。B7-H4、P53、CEA中两个或两个以上基因异常表达者与无基因或单基因异常表达者相比,在浸润深度、淋巴结转移、组织学分型及病理分期中显著相关。结论 B7-H4与胃癌的发生、发展及预后有着重要关系,联合检测B7-H4、P53、CEA能弥补单项基因检测的不足,有利于胃癌的早期诊断、生物学行为和预后的判断。
Objective To investigate the expression characteristics and clinical significance of B7-H4, P53 and CEA in gastric cancer and to find the immunological indexes for the early diagnosis of gastric cancer. Methods The expression of B7-H4, P53 and CEA in normal gastric mucosa, precancerous lesions and gastric cancer were determined by immunohistochemistry. Results B7-H4 was highly expressed in gastric cancer. The expression of B7-H4 was correlated with the depth of invasion and lymph node metastasis. The positive rate of B7-H4 in patients with survival ≤ 5 years was higher than that in patients with survival> 5 years (P <0.05). B7-H4, P53, CEA in two or more abnormal gene expression in patients with gene-null or single gene abnormal expression, depth of invasion, lymph node metastasis, histological type and pathological stage were significantly correlated. Conclusions B7-H4 plays an important role in the occurrence, development and prognosis of gastric cancer. Combined detection of B7-H4, P53 and CEA can make up for the deficiencies of single gene test, which is beneficial to the early diagnosis, biological behavior and prognosis of gastric cancer.