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Polyphenols,teroens,and resinsmake it difficult to obtain high qualitygenomic DNA from rice.Four ex-traction methods were compared inour study,and CTAB precipitationwas the most practical one.Materials used were old leavesfrom F_5 transgenic rice in Hainan andfresh leaves from F_5 transgenic rice inKunming.Procedures of the four methodswere as follows:1.Basical method:powder 1 gold or new leaves→added 2 ml ex-traction solution(100 mmol/L
Polyphenols, teroens, and resins make it difficult to obtain high quality genomic DNA from rice. Flow ex-traction methods were more in study, and CTAB precipitation was the most practical one. Materials used were old leaves from F_5 transgenic rice in Hainan and fresh leaves from F_5 transgenic rice inKunming.Procedures of the four methodswere as follows: 1.Basical method: powder 1 gold or new leaves → added 2 ml ex-traction solution (100 mmol / L