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目的应用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术及RT2 ProfilerTM PCR Array HumanTumor Metastasis(PAHS-028A)芯片筛查早期肝转移患者血清特异性蛋白及相关肿瘤基因,为大肠癌肝转移的早期诊断提供理论依据。方法应用弱阳离子蛋白质芯片(WCX2)及SELDI-TOF-MS技术检测20例大肠癌和20例大肠癌早期肝转移患者血清中蛋白的相对含量。PCR基因芯片技术对原发灶和肝转移灶组织进行差异基因筛选。结果应用SELDI-TOF-MS的3组样本质荷比在2 000～30 000间有3 774、11 851的2个蛋白峰有显著差异。应用RT2 ProfilerTM PCR Array HumanTumor Metastasis(PAHS-028A)芯片技术发现在大肠癌原发灶中表达明显高于肝转移结节的基因有:ACTB、APC、CTNNA1、ITGB3、NR4A3、MMP10、MMP11、ETV4、CTSL1、RB1、GNRH1、HPSE、CDKN2A、KISS1R、IL8RB、ITGA7、DENR、RPSA、CX-CR4、NME2、PNN、SMAD4、SRC、SSTR2、SYK、RORB、TCF20、MMP3、MYCL1、TIMP2、TIMP3、TIMP4、TRPM1;在转移灶表达明显高于原发灶的基因有:MMP9、FN1、CST7、CCL7。结论利用SELDI-TOF-MS及基因芯片技术可以为大肠癌早期肝转移的机制研究及诊断与治疗提供实验数据和资料。
Objective To screen the serum specific proteins and related oncogenes of patients with early liver metastasis by SELDI-TOF-MS and RT2 ProfilerTM PCR Array Human Tumor Metastasis (PAHS-028A) The early diagnosis of metastasis provides a theoretical basis. Methods The relative content of serum proteins in 20 cases of colorectal cancer and 20 cases of early stage liver metastasis of colorectal cancer was detected by WCX2 and SELDI-TOF-MS. PCR gene chip technology for primary tumor and liver metastasis tissue differential gene screening. Results There were significant differences in the mass-to-charge ratio of three groups with SELDI-TOF-MS between 3 000 and 30 000 with 3 774 and 11 851 peaks. The gene expression of ACTB, APC, CTNNA1, ITGB3, NR4A3, MMP10, MMP11, ETV4, MMP-9 in primary colorectal cancer was higher than that of the liver metastasis in the RT2 ProfilerTM PCR Array HumanTumor Metastasis CTSL1 RB1 GNRH1 HPSE CDKN2A KISS1R IL8RB ITGA7 DENR RPSA CXCR4 NME2 PNN SMAD4 SRC SSTR2 SYK RORB TCF20 MMP3 MYCL1 TIMP2 TIMP3 TIMP4 TIMPs TRPM1; the expression of metastasis in significantly higher than the primary tumor genes: MMP9, FN1, CST7, CCL7. Conclusion SELDI-TOF-MS and gene chip technology can provide experimental data and data for the mechanism of liver metastasis and diagnosis and treatment of colorectal cancer.