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茉莉酸是植物体内天然的调节剂,在植物生长发育过程及响应外界环境胁迫方面具有重要的作用。为了分析水稻中茉莉酸的响应基因,设计了Os PDF1.2,Os VSP2,Os JAZ8的定量PCR引物,利用100μmol/L茉莉酸和100μmol/L菲尼酮(内源茉莉酸合成抑制剂)处理的叶片为材料,分析了Os JAZ8,Os PDF1.2,Os VSP2等3个基因对茉莉酸的响应情况。结果表明,Os JAZ8,Os PDF1.2随着茉莉酸处理时间的延长,表达量逐渐升高,随着菲尼酮处理时间的延长,表达量逐渐降低,而Os VSP2则与之相反;Os JAZ8对于茉莉酸的响应较Os PDF1.2,Os VSP2这2个基因更加敏感。据此结果认为,Os JAZ8的表达量可以作为水稻叶片中茉莉酸相对含量的指标。
Jasmonic acid is a natural regulator of plant body and plays an important role in the process of plant growth and development and in response to environmental stress. In order to analyze the response genes of jasmonic acid in rice, quantitative PCR primers of Os PDF1.2, Os VSP2 and Os JAZ8 were designed and treated with 100 μmol / L jasmonic acid and 100 μmol / L phenidone (endogenous inhibitor of jasmonic acid synthesis) Of leaves as the material, the analysis of Os JAZ8, Os PDF1.2, Os VSP2 three genes response to jasmonic acid. The results showed that the expression level of Os JAZ8 and Os PDF1.2 gradually increased with the prolonging of jasmonic acid treatment time, and gradually decreased with the increase of the time of phenidone treatment, whereas Os VSP2 was the opposite. Os JAZ8 The response to jasmonic acid was more sensitive than Os PDF1.2 and Os VSP2. According to the results, Os JAZ8 expression can be used as an indicator of the relative content of jasmonic acid in rice leaves.