慢性肾衰患者血清及尿液γ-干扰素的检测及其意义

• 作 者: 刘华锋 陈群 唐蓉 王胜春 麦伟民 汤斌 邱丽清 谭惠莲
• 机 构: 广东医学院肾脏病研究所
• 出 处: 中国实用内科杂志
• 发表日期: 1998年12期
• 关键词: 慢性肾衰患者 慢性肾功能衰竭 γ-干扰素 γ－干扰素 双抗体夹心法 肾小球滤过功能 细胞因子 肾脏损伤 高血压性肾硬化 肾小管上皮细胞

采用ＥＬＩＳＡ双抗体夹心法检测７１例慢性肾衰（ＣＲＦ）患者及４０例健康成人血及尿的γ－干扰素（ＩＦＮ－γ）水平。结果显示：ＣＲＦ患者血清及尿液ＩＦＮ－γ含量分别为（６２６２±２．６８）ｎｇ／Ｌ和（４３．０１±２．５７）ｎｇ／Ｌ，显著低于正常对照组，［（１０５±７．１０）ｎｇ／Ｌ和（７５±６．１４）ｎｇ／Ｌ］（Ｐ＜０００１），但与尿素氮（ＢＵＮ）及血清肌酐（Ｓｃｒ）无显著相关性（血清及尿ＩＦＮ－γ与ＢＵＮ、Ｓｃｒ相关系数分别是ｒ＝０．２４２５、０３０５６、０１５３３和０１７５０，Ｐ＞００５）。结论：ＩＦＮ－γ作为一种细胞因子参与肾脏病发生、发展过程中的网络调节，血清及尿液中ＩＦＮ－γ水平受到机体Ｔ淋巴细胞及ＮＫ细胞数量及功能影响。

The levels of IFN-γin blood and urine of 71 patients with chronic renal failure (CRF) and 40 healthy adults were detected by ELISA double antibody sandwich method. The results showed that the levels of IFN-γ in serum and urine of patients with CRF were (6262 ± 2.68) ng / L and (43.01 ± 2.57) ng / L, respectively, which were significantly lower than those in the control group [[ 105 ± 7.10) ng / L and (75 ± 6.14) ng / L] (P <0001), but no significant correlation with BUN and Scr (serum and urine The correlation coefficients of IFN-γ, BUN and Scr were r = 0.2425,03056,01533 and 01750, respectively, P> 005). CONCLUSION: IFN-γ is involved as a cytokine in the development of nephrotic syndrome. Network regulation of IFN-γ in serum and urine is influenced by the number and function of T lymphocytes and NK cells.