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目的:研究明日叶(Angelica keiskei Koidz)查尔酮对小鼠肝癌细胞PCNA和BCL-2蛋白表达的影响。方法:将50只皮下接种肝癌H22细胞株的小鼠随机分为5组,每组10只。高、中、低剂量组分别每日经口灌胃给予40、20、5mg/kg的查尔酮,肿瘤对照组给予等量生理盐水,连续10d,环磷酰胺组隔天腹腔注射环磷酰胺20mg/kg。取肝癌组织用四甲基偶氮噻唑蓝(MTT)法测各组小鼠肝癌细胞增殖活性,免疫组化法检测各组肝癌细胞增殖细胞核抗原(PCNA)和凋亡相关蛋白BCL-2表达水平。结果:高剂量查尔酮组和肿瘤对照组的肝癌细胞增殖活性分别为(0.716±0.018)和(1.135±0.032),差别有显著性(P<0.05)。高剂量组PCNA和BCL-2蛋白表达率分别为28.33%和16.77%,肿瘤对照组分别为72.77%和65.17%,差异均有显著性(P<0.05)。结论:查尔酮可降低小鼠肝癌细胞PCNA和BCL-2表达水平,对肝癌细胞增殖有一定抑制作用。
Objective: To investigate the effect of chalcone from Angelica keiskei Koidz on the expression of PCNA and BCL-2 in mouse hepatoma cells. Methods: Fifty mice subcutaneously inoculated with H22 cell line were randomly divided into 5 groups with 10 in each. High, medium and low dose groups were orally administered daily oral gavage 40,20,5mg / kg, the control group was given an equal amount of saline, continuous 10d, cyclophosphamide group intraperitoneal injection of cyclophosphamide 20 mg / kg. The proliferation of hepatocellular carcinoma cells in each group was detected by MTT method. The expression of proliferating cell nuclear antigen (PCNA) and apoptosis related protein BCL-2 in each group were detected by immunohistochemistry . RESULTS: The proliferation activity of hepatocarcinoma cells in high-dose chalcone group and tumor control group were (0.716 ± 0.018) and (1.135 ± 0.032), respectively, with significant difference (P <0.05). The protein expression rates of PCNA and BCL-2 in the high-dose group were 28.33% and 16.77%, respectively, and those in the tumor control group were 72.77% and 65.17%, respectively. The difference was significant (P <0.05). Conclusion: Chalcone can reduce the expression of PCNA and BCL-2 in mouse hepatoma cells and inhibit the proliferation of hepatoma cells.