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目的：癫痫是一种神经系统常见病，其发病机制较为复杂。近年来研究发现，在许多化学药物及电刺激所造成的癫痫发作模型中均可诱导热休克蛋白７０（ＨＳＰ７０）的合成。表明ＨＳＰ７０与癫痫发作有着密切的关系。方法：选择针对ＨＳＰ７０的鼠单克隆特异抗体，采用免疫组织化学法对海人酸（ＫＡ）诱导ＳＤ大鼠癫痫发作后ＨＳＰ７０表达进行了观察，并就ＭＫ－８０１对ＨＳＰ７０表达的影响做了初步探讨。结果：ＫＡ注射后２４ｈ，ＨＳＰ７０免疫反应阳性细胞主要分布在边缘结构，多数皮质区也有表达；预先用ＭＫ－８０１后，全部大鼠均没有出现明显惊厥行为，多数皮质区及丘脑区ＨＳＰ７０表达消失，但在齿状回，ＣＡ３区仍有高表达。结论：ＭＫ－８０１具有抗惊厥及神经保护作用，其对某些脑区ＨＳＰ７０表达的抑制作用显示出它对ＫＡ引起的同步性痫样放电活动的破坏
Objective: Epilepsy is a common disease of the nervous system, its pathogenesis is more complicated. In recent years, the study found that many chemical drugs and electrical stimulation induced seizures model induced heat shock protein 70 (HSP70) synthesis. HSP70 and epilepsy have a close relationship. Methods: The murine monoclonal antibody specific to HSP70 was selected and the expression of HSP70 was detected by immunohistochemistry after KA-induced epileptic seizures in SD rats. The effects of MK-801 on the expression of HSP70 Discussion. Results: The expression of HSP70 immunoreactive cells was mainly located in the marginal structure at 24 h after KA injection, and most of the cortex regions were also expressed. After the MK-801 was pre-treated, no obvious convulsion was found in all the rats, and the expression of HSP70 disappeared in most cortex and thalamus , But in the dentate gyrus, CA3 area is still high expression. CONCLUSION: MK-801 has anticonvulsant and neuroprotective effects. Its inhibitory effect on the expression of HSP70 in some brain regions shows its disruption of synchronous epileptiform discharge caused by KA