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为了探讨亚精胺提高大豆幼苗抗盐性的内在机理,以郑豆30为材料,采用沙培法,研究了在100 mmol·L~(-1)盐胁迫下,不同浓度(0.1,0.5,1.0和1.5 mmol·L~(-1))的亚精胺对大豆幼苗叶片光合特性和抗氧化代谢的影响。结果表明:在盐胁迫下,大豆幼苗相对干物质增长速率(RDMIR)、抗氧化酶(SOD、POD、CAT和APX)活性和光合参数(Pn、Ci、Tr和Gs)下降,相对电解质渗透率(RELR)、超氧阴离子产生速率(O~-_2·)、过氧化氢(H_2O_2)和丙二醛(MDA)含量上升,但经过不同浓度的亚精胺处理后,大豆幼苗叶片RELR、O~-_2·、H_2O_2和MDA含量下降,而RDMIR、抗氧化酶活性和光合参数却上升,且以0.5 mmol·L~(-1)的Spd处理效果最显著。这些结果表明亚精胺能通过提高大豆幼苗叶片的抗氧化酶活性而降低幼苗叶片的O~-_2·产生速率、H_2O_2和MDA含量,通过提高光合相关参数指标提高幼苗叶片的光合效率,从而减轻盐胁迫的伤害,促进大豆幼苗生长。
In order to investigate the intrinsic mechanism of spermidine to improve the salt tolerance of soybean seedlings, the effects of different concentrations of 0.1, 0.5, Effects of 1.0 and 1.5 mmol·L -1 Spermidine on Photosynthetic Characteristics and Antioxidative Metabolism in Soybean Seedling Leaves. The results showed that the relative dry matter growth rate (RDMIR), antioxidant enzyme activities (SOD, POD, CAT and APX) and photosynthetic parameters (Pn, Ci, Tr and Gs) of soybean seedlings decreased, and the relative electrolyte permeability (RELR), superoxide anion production rate (O ~ -_2 ·), hydrogen peroxide (H_2O_2) and malondialdehyde (MDA) content increased, but after different concentrations of spermidine treatment, soybean seedling leaves RELR, O ~ 2 · H 2 O 2 and MDA content decreased, while the activities of RDMIR, antioxidant enzymes and photosynthetic parameters increased, and the effect of Spd was the most significant at 0.5 mmol·L -1. These results indicate that spermidine can reduce the O 2 production rate, H 2 O 2 and MDA content of seedling leaves by increasing the antioxidant enzyme activity of soybean seedling leaves, and improve the photosynthetic efficiency of seedling leaves by increasing the related parameters of photosynthesis, Salt stress damage, promote soybean seedling growth.