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目的:研究牙龈紫质单胞菌脂多糖(Pg-LPS)诱生IL-1、TNF、PGE和激活破骨细胞的活性,了解破骨细胞激活机制。方法:用酚水法制备Pg-LPS,采用层析法分离Pg-LPS诱生的IL-1、TNF和PGE,用原子吸收光谱法测定Ca~(2+)离子浓度,用组织化学法检测牙周膜中酸性磷酸酶和碳酸酐酶。结果:Pg-LPS能刺激人外周血单个核细胞(PBMC)或人牙周组织细胞分泌IL-1、TNF和PGE。这些细胞因子的产量在一定范围内随Pg-LPS浓度(0.5-5.0mg/L)增高而增加。IL-1、TNF和PGE能促进SD大鼠头盖骨Ca~(2+)离子的释放,但以PGE的活性最强。Pg-LPS注射的SD大鼠牙周膜中酸性磷酸酶和碳酸酐酶数量明显升高(P<0.01)。SD大鼠牙周膜中活化的破骨细胞数量随Pg-LPS注射次数增加而明显增多(P<0.01),但由于未活化破骨细胞数量也随之增多而使破骨细胞活化率稳定在65％左右。结论:Pg-LPS具有很强的诱导人PBMC和人牙周组织细胞产生IL-1、TNF或PGE的作用,这种作用在一定的范围内呈剂量依赖型。Pg-LPS可有效地激活破骨细胞,其激活机制可能与碱性磷酸酶和碳酸酐酶增多有关。
OBJECTIVE: To study the activity of IL-1, TNF, PGE and activation of osteoclasts induced by Pg-LPS, and to understand the mechanism of osteoclast activation. Methods: Pg-LPS was prepared by phenol water method. IL-1, TNF and PGE induced by Pg-LPS were separated by chromatographic method. The concentration of Ca 2+ was determined by atomic absorption spectrometry Periodontal ligament acid phosphatase and carbonic anhydrase. RESULTS: Pg-LPS stimulated the secretion of IL-1, TNF and PGE from human peripheral blood mononuclear cells (PBMCs) or human periodontal tissues. The production of these cytokines increased to a certain extent with the increase of Pg-LPS concentration (0.5-5.0mg / L). IL-1, TNF and PGE can promote the release of Ca2 + in skull of SD rats, but the activity of PGE is the strongest. The numbers of acid phosphatase and carbonic anhydrase in periodontal ligament of Pg-LPS injected SD rats were significantly increased (P <0.01). The number of osteoclasts activated in the periodontal ligament of SD rats increased significantly with the increase of Pg-LPS injection times (P <0.01), but the activation rate of osteoclasts was stable at the increase of the number of non-activated osteoclasts About 65%. CONCLUSION: Pg-LPS has a strong effect of inducing IL-1, TNF or PGE production in human PBMCs and human periodontal cells in a dose-dependent manner. Pg-LPS can effectively activate osteoclasts, the activation mechanism may be related to increased alkaline phosphatase and carbonic anhydrase.