【摘 要】
：
通过系统探讨不同孤雌激活方法对猪卵母细胞体外激活后发育效果的影响,比较了乙醇(Ethanol,EH)、离子霉素(Ionomycin,Ion:5μmol/L)、氯化锶(Strontium chloride hexahydrate
【基金项目】
：
国家高技术研究发展计划(863计划);
论文部分内容阅读
通过系统探讨不同孤雌激活方法对猪卵母细胞体外激活后发育效果的影响,比较了乙醇(Ethanol,EH)、离子霉素(Ionomycin,Ion:5μmol/L)、氯化锶(Strontium chloride hexahydrate,Sr2+:10 mmol/L)、6-二甲氨基嘌呤(6-dimethylaminopurine,6-DMAP:2 mmol/L)和放线茵酮(cycloheximide,CHX:10 mg/L)对猪卵母细胞激活发育的效果.结果表明:(1)9%EH激活处理10 min效果好于15 min;(2)使用9%EH激活处理10 min,再结合CHX、6-DMAP、Sr2+、CHX+Sr2+、Sr2++6-DMAP、CHX+6-DMAP或CHX+6-DMAP+Sr2+组处理3～4 h,以EH+6-DMAP组效果最好,分裂率及囊胚率分别达到82.86%和22.86%;(3)在使用化学激活(Ion+6-DMAP组和9%乙醇10 min+6-DMAP组)和电激活(50 V/mm,50 μs,2 t)的方法中,Ion法激活猪卵母细胞效果较好,囊胚率达到37.50%;(4)卵母细胞包被的卵丘细胞层数不同对卵母细胞成熟激活有显著的影响,卵丘细胞层数4～6层和多于6层的卵丘-卵母细胞复合体孤雌激活的分裂率和囊胚率分别为(68.99%,32.56%)和(75.36%,37.68%),2组之间差异不显著(P0.05);但其显著高于其他组(P
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