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为了建立磷酸己糖异构酶（ＰＨＩ） 的酶偶联测定法， 并观察其临床应用价值， 根据ＰＨＩ可催化６－磷酸果糖转化为６－磷酸葡萄糖（Ｇ６Ｐ）， 在６－磷酸葡萄糖脱氢酶催化下， ６－磷酸葡萄糖转化为６－磷酸葡萄糖酸， 同时使ＮＡＤＰ还原为ＮＡＤＰＨ， 在３４０ ｎｍ 处吸光度变化速率与ＰＨＩ的活性成正比。方法的批内ＣＶ＜ １．２％ ， 批间ＣＶ＜ １．５％ ， 回收率９８％ ～１０２％ ， 线性达１０００ Ｕ／Ｌ。健康对照组ＰＨＩ９５％ 可信限为２０～８９ Ｕ／Ｌ。取ＰＨＩ１５０ Ｕ／Ｌ作为肺癌与其他肺部疾病鉴别诊断指标， 鉴别诊断效率达０．８０５。
In order to establish an enzyme conjugate assay for phosphohexose isomerase (PHI) and observe its clinical application value, PHI can catalyze the conversion of 6-phosphorylated fructose to glucose-6-phosphate (G6P), which dehydrogenates glucose at 6-phosphate. Enzymatically, glucose 6-phosphate is converted to 6-phosphogluconate and NADP is reduced to NADPH. The rate of change in absorbance at 340 nm is proportional to the activity of PHI. The method has a batch CV <1.2%, an inter-assay CV < 1.5%, a recovery of 98% ~ 102%, and a linearity of 1000 U/L. The PHI 95% confidence limit of the healthy control group was 20-89 U/L. PHI150 U/L was used as a differential diagnosis index for lung cancer and other lung diseases. The differential diagnosis efficiency was 0.805.